Bioassay Methods Group
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Our group investigates fundamental biological processes by means of spectroscopy, electrochemistry, calorimetry, and molecular biology measurements to develop new and improved techniques, methodologies, and standards; uses state-of-the-art techniques and methods to explore and resolve measurement issues in molecular biology, nanobiotechnology, immunology, biophysical chemistry, molecular spectrometry and surface chemistry; works with users and manufacturers of analytical and bioanalytical instruments to assess method performance and to determine and address existing and future standards needs ranging from device calibration and instrument performance to the development of biological reference materials and procedures; works cooperatively with other government agencies and laboratories to improve the quality and reliability of their measurements in the biological sciences; and supports a broad range of health, defense, environmental, and energy research related activities and customers.
Projects/Programs
Precision Medicine for Cancer Diagnostics—The conventional approach to medicine is rapidly changing to a data-driven strategy in which therapies are individually targeted to a patient based on genome, physiology, environment, or lifestyle...
Measurement of Low Frequency Mutations in Circulating Tumor DNA (ctDNA) through Interlaboratory Testing—A very exciting development in the diagnosis and therapeutic monitoring of cancer is the discovery that blood and urine contain nucleic acids derived from cancer cells that can be used to as biomarkers...
Quantitative Flow Cytometry—We work in collaboration with other government agencies, private industry, academia, professional societies, and other national metrology institutes to ensure that flow cytometry measurements are …
Thermodynamics of Enzyme-catalyzed Reactions—To develop the thermodynamic data and methodology required for effective bioprocess engineering...
Quantification of Cells with Specific Phenotypic Characteristics—Flow cytometers are versatile instruments that have been successfully used to enumerate cell subsets in infectious diseases (e.g., HIV) and to diagnose malignancies through changes in the …
News & Publications
- Assignment of the Number of Equivalent Reference Fluorophores to Dyed Microspheres
- Automated Spectral Smoothing with Spatially Adaptive Penalized Least-Squares
- Certified DNA Reference Materials to Compare HER2Gene Amplification Measurements Using Next-Generation Sequencing Method
- Enzyme Catalyzed Reactions in Biocalorimetry: Foundations and Contemporary Approaches, Biocalorimetry: Foundations and Contemporary Approaches, CRC Press
- NIST and Frederick National Laboratory for Cancer Research Help Ensure Accurate Clinical Measurements of HER2 Breast Cancer Gene
- Standards for Cell Line Authentication and Beyond
- Relative intensity correction of Raman spectrometers: NIST SRMs 2241 through 2243 for 785 nm, 532 nm, and 488 nm/514.5 nm excitation
- Use of Standard Reference Material 2242 (Relative Intensity Correction Standard for Raman Spectroscopy) for Microarray Scanner Qualification