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Jamie Almeida (Fed)

Research Biologist

The Biomarker and Genomic Sciences Group focuses on a variety of standards to improve measurements and diagnostics in the health care field including characterization of gene delivery systems, cancer biomarker standards and cell line authentication methods.

  • Consensus Standards
    • Serve as a convenor of the International Organization for Standardization (ISO) Technical Committee 276 (TC 276)  Biotechnology, Subcommittee 1 (SC1) Analytical Methods, Working Group 3 (WG3) Nucleic Acids.
    • Serve as a co-lead in the ISO TC 276/SC1/WG1/16921 document series focusing on gene delivery systems terminology, viral vector quantification, and lipid nanoparticle characterization and quantification.
    • Serve as an active member of the ATCC/ANSI Standards Development Organization. Updating ATCC/ANSI ASN-0003-2015: Species-Level Identification of Animal Cells through Mitochondrial Cytochrome c Oxidase Subunit 1 (CO1) DNA Barcodes.
  • Reference Materials
    • Performed genomic measurements using droplet digital PCR and qPCR to certify the following cancer biomarker reference materials:  Standard Reference Material 2373 (certified for HER2 copy number) and Reference Material 8366 (certified for MET and EGFR copy number).
    • Characterization of Reference Material 8399 for a mouse allelic ladder to be used for allele call determination in mouse cell line authentication using short tandem repeat genotyping (in progress).
  • Consortium Work
    • Serve as a co-lead for the Gene Delivery Systems Working Group in the Flow Cytometry Standard Consortium. Please visit the consortium page for more information.
      • Work with stakeholders to join the consortium and contribute gene delivery system materials, instrumentation, and expertise.
      • Organize and plan the first interlaboratory study to focus on physical titer measurements including genome titer, capsid titer, and particle concentration.
        • Develop protocols to reduce variability and improve reproducibility for physical titer measurements for gene delivery systems.
  • Research and Development
    • Coordinate the gene delivery systems effort in the Biosystems and Biomaterials Division. Please visit the program page for more information.

      Illustration of adenovirus serotype 5 particle
      Illustration of adenovirus serotype 5 particle
    • Working with NIST colleagues to improve functional measurements of adenovirus in cell-based assays using live cell microscopy.
    • Collaborate with NIST IT group to develop AI platform to be used for plaque predications and optimized counting techniques.
    • Collaborate with NIST colleagues to collect metabolomics measurements on serum-free HEK293T cells to determine cell health metrics when preparing cells for functional assays in order to improve reproducibility.

 

 

  • Cell Line Authentication
    • Co-inventor of the method for mouse cell line authentication using a short tandem repeat (STR) targeted multiplex PCR assay.
      • US Patent 9,556,482
      • US Patent 10,745,753
    • Organized and led the Mouse Cell Line Authentication Consortium to validate 19 mouse STR markers for genotyping mouse cell lines for identification. The outcome of the consortium led to the development of a mouse STR profile repository on the NCBI BioSample Database and publication of the interlaboratory results from participating laboratories. 

Awards

  • NIST William P. Slichter Award, "for Organization of a consortium to validate a DNA genotyping method, database, and materials to authenticate the identity of mouse cell lines", 2021
  • NIST Judson C. French Award, “for development of copy number standard reference material SRM 2373 for diagnosing cancer”, 2016
  • US Department of Commerce Bronze Medal Award, “for development of a method for mouse cell line authentication”, 2015
  • NIST Safety Award, “for developing and promoting biosafety training”, 2012

Selected Publications

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines

Author(s)
Jamie L. Almeida, Aleksandra Dakic, Karin Kindig, Maikan Kone, Deborah L. Letham, Scott Langdon, Ruth Peat, Jayamalini Holding-Pillai, Erin Hall, Mark Ladd, Megan Shaffer, Heath Berg, Jinliang Li, Georges Wigger, Steven P. Lund, Carolyn R. Steffen, Barbara Fransway, Bob Geraghty, Manuela Natoli, Beth Bauer, Susanne M. Gollin, Dale Lewis, Yvonne A. Reid
The Consortium for Mouse Cell Line Authentication was formed to validate Short Tandem Repeat (STR) markers for intraspecies identification of mouse cell lines

Standards for cell line authentication and beyond

Author(s)
Jamie L. Almeida, Kenneth D. Cole, Anne L. Plant
Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat profiling) has been the subject of a

Publications

Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines

Author(s)
Jamie L. Almeida, Aleksandra Dakic, Karin Kindig, Maikan Kone, Deborah L. Letham, Scott Langdon, Ruth Peat, Jayamalini Holding-Pillai, Erin Hall, Mark Ladd, Megan Shaffer, Heath Berg, Jinliang Li, Georges Wigger, Steven P. Lund, Carolyn R. Steffen, Barbara Fransway, Bob Geraghty, Manuela Natoli, Beth Bauer, Susanne M. Gollin, Dale Lewis, Yvonne A. Reid
The Consortium for Mouse Cell Line Authentication was formed to validate Short Tandem Repeat (STR) markers for intraspecies identification of mouse cell lines

Patents (2018-Present)

Figure 1

Mouse Cell Line Authentication

NIST Inventors
Jamie Almeida
A method, kit and primers for determining alleles present in a DNA sample that is sourced from either a mouse or a cell line derived from a mouse. A set of STR marker loci are selected that can be amplified together in a multiplex amplification reaction. The set of STR marker loci are selected from

Mouse Cell Line Authentication

NIST Inventors
Jamie Almeida and Kenneth D. Cole
A method, kit and primers for determining alleles present in a DNA sample that is sourced from either a mouse or a cell line derived from a mouse. A set of STR marker loci are selected that can be amplified together in a multiplex amplification reaction. The set of STR marker loci are selected from
Created October 9, 2019, Updated September 30, 2025
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