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Jamie Almeida (Fed)
The Biomarker and Genomic Sciences Group focuses on a variety of standards to improve measurements and diagnostics in the health care field including characterization of viral vector reference materials, cancer biomarker standards and cell line authentication methods.
Research projects
- Viral Vector Reference Material Characterization
- Lead the Adenovirus serotype 5 (Ad5) viral vector RM characterization and assay development.
- Organize Research Cooperative Agreement (RCA) with ATCC for testing new Ad5 materials as candidate reference material and perform bridging studies with previous Ad5 RM.
- Organize participants for interlaboratory measurements to characterize candidate Ad5 material.
- Optimize and improve infectivity measurements for Ad5 including tissue culture infectious dose 50 (TCID50), plaque assay, and immunocytochemistry methods by incorporating live cell imaging.
- Lead the Adenovirus serotype 5 (Ad5) viral vector RM characterization and assay development.
- Cell Line Authentication
- Short tandem repeat (STR) targeted multiplex PCR assay development for African green monkey, mouse, and Chinese hamster cell lines.
- Organized and led the Mouse Cell Line Authentication Consortium to validate 19 mouse STR markers for genotyping mouse cell lines for identification. The outcome of the consortium led to the development of a mouse STR profile repository on the NCBI BioSample Database and publication of the interlaboratory results from 12 participating laboratories.
- Early Detection Research Network (part of the National Cancer Institute)
- Methylation of biomarkers for early detection of cancer.
- Develop and optimize droplet digital PCR assays to measure methylation for these biomarkers.
- Develop, characterize, and perform stability study testing on reference materials to measure copy number for HER2 (SRM 2373) and MET/EGFR (RM8366).
- Reference Materials
- Quantitative PCR and droplet digital PCR measurements for Standard Reference Material 2373 (certified for HER2 copy number) and Reference Material 8366 (certified for MET and EGFR copy number).
- Development of a reference material for a mouse allelic ladder (RM 8399) to be used for allele call determination in mouse cell line authentication.
- Characterization of a reference material for methylation in cancer biomarkers.
Patents
Awarded
- Almeida, Jamie L., Cole, Ken D. Mouse Cell Line Authentication. US 10,745,753 B2 United States Patent and Trademark Office, 18 August 2020.
- Almeida, Jamie L., Cole, Ken D. Mouse Cell Line Authentication. US 9,556,482 B2, United States Patent and Trademark Office, 31 January 2017.
Awards
-
NIST William P. Slichter Award, "for Organization of a consortium to validate a DNA genotyping method, database, and materials to authenticate the identity of mouse cell lines", 2021
- NIST Judson C. French Award, “for development of copy number standard reference material SRM 2373 for diagnosing cancer”, 2016
- US Department of Commerce Bronze Medal Award, “for development of a method for mouse cell line authentication”, 2015
- NIST Safety Award, “for developing and promoting biosafety training”, 2012
