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Jamie Almeida (Fed)
Research Biologist
The Biomarker and Genomic Sciences Group focuses on a variety of standards to improve measurements and diagnostics in the health care field including characterization of viral vector reference materials, cancer biomarker standards and cell line authentication methods.
Jamie aids in the coordination of the gene delivery systems effort in the Biosystems and Biomaterials Division. Please visit the program page for more information.
Research projects
- Viral Vector Reference Material Characterization
- Lead the Adenovirus serotype 5 (Ad5) viral vector RM characterization and assay development.
- Organize Research Cooperative Agreement (RCA) with ATCC for testing new Ad5 materials as candidate reference material and perform bridging studies with previous Ad5 RM.
- Optimize and improve functional titer measurements for Ad5 reference materials.
Visible plaques form in HEK293 cells infected with Adenovirus. Incorporate live cell imaging to monitor plaque formation and enumeration. Image courtesty of Ed Kwee.
- Lead the Adenovirus serotype 5 (Ad5) viral vector RM characterization and assay development.
- Cell Line Authentication
- Short tandem repeat (STR) targeted multiplex PCR assay development for non-human cell line authentication.
- Organized and led the Mouse Cell Line Authentication Consortium to validate 19 mouse STR markers for genotyping mouse cell lines for identification. The outcome of the consortium led to the development of a mouse STR profile repository on the NCBI BioSample Database and publication of the interlaboratory results from 12 participating laboratories.
- Early Detection Research Network (part of the National Cancer Institute)
- Methylation of biomarkers for early detection of cancer.
- Develop and optimize droplet digital PCR assays to measure methylation for these biomarkers.
- Develop, characterize, and perform stability study testing on reference materials to measure copy number for HER2 (SRM 2373) and MET/EGFR (RM8366).
- Reference Materials
- Quantitative PCR and droplet digital PCR measurements for Standard Reference Material 2373 (certified for HER2 copy number) and Reference Material 8366 (certified for MET and EGFR copy number).
- Development of a reference material for a mouse allelic ladder (RM 8399) to be used for allele call determination in mouse cell line authentication (in progress).
Awards
-
NIST William P. Slichter Award, "for Organization of a consortium to validate a DNA genotyping method, database, and materials to authenticate the identity of mouse cell lines", 2021
- NIST Judson C. French Award, “for development of copy number standard reference material SRM 2373 for diagnosing cancer”, 2016
- US Department of Commerce Bronze Medal Award, “for development of a method for mouse cell line authentication”, 2015
- NIST Safety Award, “for developing and promoting biosafety training”, 2012
