Skip to main content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Measurement of Oxidatively Induced DNA Damage in Caenorhabditis elegans with High-Salt DNA Extraction and Isotope-Dilution Mass Spectrometry



Leona D. Scanlan, Pawel Jaruga, Sanem Hosbas Coskun, Christopher M. Sims, Shannon Hanna, Jamie L. Almeida, David N. Catoe, Bryant C. Nelson, M Miral Dizdar, Erdem Coskun


Caenorhabditis elegans (C. elegans) is used as a medical and systems toxicity model organism for environmental and developmental assays that include high-throughput methods and genetic studies. However, little is known about background levels of oxidatively induced DNA damage in the nematode or how culturing methods may affect DNA damage levels. Furthermore, C. elegans physiology makes it difficult to extract large amounts (> 50 µg) of high-quality genomic DNA. We therefore developed and optimized a DNA extraction method based on enzymatic digestion and high-salt phase separation that resulted in efficient extraction of > 50 µg DNA from 250000 to 500000 nematodes. Extracted DNA showed suitable RNA levels (
Analytical Chemistry
Created August 27, 2019, Updated October 8, 2019