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Standards for cell line authentication and beyond

Published

Author(s)

Jamie L. Almeida, Kenneth D. Cole, Anne L. Plant

Abstract

Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat profiling) has been the subject of a comprehensive standard (ASN-0002). Here we discuss the power of this document, and why standards such as this are so critical for establishing the consensus technical criteria and practices that can enable progress in the fields of research that use cell lines. We also examine other methods that could be used for authentication, and discuss how a combination of methods could be used in a holistic fashion to assess various critical aspects of the quality of cell lines.
Citation
Plos Biology
Volume
14
Issue
6

Keywords

authentication, cell line, short tandem repeat, STR, contamination, identity, reproducibility

Citation

Almeida, J. , Cole, K. and Plant, A. (2016), Standards for cell line authentication and beyond, Plos Biology, [online], https://doi.org/10.1371/journal.pbio.1002476 (Accessed April 21, 2021)
Created June 14, 2016, Updated November 10, 2018