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Standards for cell line authentication and beyond



Jamie L. Almeida, Kenneth D. Cole, Anne L. Plant


Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat profiling) has been the subject of a comprehensive standard (ASN-0002). Here we discuss the power of this document, and why standards such as this are so critical for establishing the consensus technical criteria and practices that can enable progress in the fields of research that use cell lines. We also examine other methods that could be used for authentication, and discuss how a combination of methods could be used in a holistic fashion to assess various critical aspects of the quality of cell lines.
Plos Biology


authentication, cell line, short tandem repeat, STR, contamination, identity, reproducibility


Almeida, J. , Cole, K. and Plant, A. (2016), Standards for cell line authentication and beyond, Plos Biology, [online],, (Accessed June 17, 2024)


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Created June 13, 2016, Updated October 12, 2021