My research mostly focuses on using quantitative flow cytometry to measure clinically and biomanufacturing relevant biological substances, cells, extracellular vesicles/exosomes, virus, proteins, DNA and RNA. Current effort includes development of additional reference fluorophore solutions and reference cell materials for standardizing flow cytometer intensity scale and performance characteristics in the unit of the equivalent number of reference fluorophores (ERF) and building sufficient measurement assurance in counting of bio-entities and biomarker expression analysis using flow cytometry. We provide an ERF value assignment service to the manufacturers of cytometer calibration particles under the flow cytometry quantitation consortium. The application of cell reference materials facilitates the quantitative measure of unknown biomarker expression in the unit of antibodies bound per cell (ABC), independent of the flow cytometers used. Additionally, we work closely with other international metrological institutions for the development of cell and other bio-entity counting reference materials. Examples of current projects include 1) characterization of biological reference materials including cell lines, engineered cell lines, primary cells, lyophilized cells, and exosomes; 2) quantifying expression of cell surface and intracellular biomarkers by estimating the antibodies bound per cell; 3) enumeration of cells with specific phenotypic characteristics and function, e.g. CD34+ stem cells and cytokine producing T cells; 4) harmonizing flow cytometry data across different cytometer platforms through panel design, antibody titration, instrument calibration, reference cell controls, and high dimensional data analysis.