My research mostly focuses on using quantitative flow cytometry to measure clinically relevant biological substances, cells, proteins, DNA and RNA. Current effort includes the production of NIST SRM 1934 and establishing a standard operating procedure for assignment of the equivalent number of reference fluorophores (ERF) to calibration microparticles. The use of these calibration microparticles with assigned ERF values under a NIST measurement service enables the standardization of fluorescence intensity scales of flow cytometers. Additionally, my team focuses on characterizing expression levels of biological cell reference materials. The application of cell reference materials facilitates the quantitative measure of unknown biomarker expression in the unit of antibodies bound per cell (ABC), independent of the flow cytometers used. Examples of current projects include quantifying expression of ZAP-70, a prognostic marker of Chronic Lymphocytic Leukemia and developing multiplexed assays on cellular subsets to correlate microRNA expression level with intracellular cytokine expression.
Memberships and Committees:
NIST-NRC and NIH/NIST NRC Postdoctoral Fellowship Opportunities: