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Lili Wang

Research Interests:

My research mostly focuses on using quantitative flow cytometry to measure clinically relevant biological substances, cells, proteins, DNA and RNA. Current effort includes the production of NIST SRM 1934 and establishing a standard operating procedure for assignment of the equivalent number of reference fluorophores (ERF) to calibration microparticles. The use of these calibration microparticles with assigned ERF values under a NIST measurement service enables the standardization of fluorescence intensity scales of flow cytometers. Additionally, my team focuses on characterizing expression levels of biological cell reference materials. The application of cell reference materials facilitates the quantitative measure of unknown biomarker expression in the unit of antibodies bound per cell (ABC), independent of the flow cytometers used. Examples of current projects include quantifying expression of ZAP-70, a prognostic marker of Chronic Lymphocytic Leukemia and developing multiplexed assays on cellular subsets to correlate microRNA expression level with intracellular cytokine expression.

Memberships and Committees:

  • CDRH/FDA Hematology and Pathology Devices Panel Advisory Committee, Consultant, 2011- 2019
  • JCTLM Team Leader on Blood Cell Counting and Typing, 2009-present
  • ISAC Standards Committee Fluorescence Calibration Task Force, Co-chair, 2009-present
  • ICCS Quality and Standards Committee, 2016-present
  • USP CD34 – Positive Cells Expert Panel, 2010-2016
  • IFCC cTnI Standardization Working Group, 2007-present


  • CSTL Technical Achievement Award (2004)
  • CSTL Director's Cash-in-a-Flash Award (2007)
  • Emerald Honor Award from Minorities in Research/Career Communications Group (2007)
  • Department of Commerce Bronze Award (2015)

Selected Publications:

  1. Quantitative Flow Cytometry Measurements in Antibodies Bound per Cell Based on a CD4 Reference. Curr. Protoc. Cytom. 75:1.29.1-1.29.14 (2016).
  2. Consistent, multi-instrument single tube quantification of CD20 in antibody bound per cell based on CD4 reference. Cytometry part B (Clinical Cytometry), doi: 10.1002/cyto.b.21253.
  3. Determination of CD4+ Cell Count per µL in Reconstituted Lyophilized Human PBMC Pre-labelled with Anti-CD4 FITC Antibody. Cytometry 87A, 244-253 (2015).
  4. Quantification of Cells with Specific Phenotypes II: Determination of CD4 Expression Level on Lyophilized Human PBMC Surface Labeled with Anti-CD4 FITC Antibody. Cytometry 87A, 254-261 (2015).
  5. Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry. Clin Proteomics 11:43 (2014).
  6. Quantifying CD4 Receptor Density on Human T Lymphocytes Using Multiple Reaction Monitoring Mass Spectrometry. Anal Chem 85, 1773-1777 (2013).
  7. Breast cancer biomarker measurements and standards: progress on Her2. Proteomics Clin. Appl. 7, 17-29 (2013).
  8. Meiyao Wang, Hua-Jun He, I.V. Turko, K.W. Phinney, and L. Wang. Quantifying CD4 Receptor Density on Human T Lymphocytes Using Multiple Reaction Monitoring Mass Spectrometry. Anal Chem 85, 1773-1777 (2013).
  9. NIST/ISAC standardization study: variability in assignment of intensity values to fluorescence standard beads and in cross calibration of standard beads to hard dyed beads. Cytometry Part A, 81A, 785-796 (2012).
  10. Human CD4+ Lymphocytes for Antigen Quantification: Characterization Using Conventional Flow Cytometry and Mass Cytometry. Cytometry Part A 81A, 567-575 (2012).
  11. A Model for Harmonizing Flow Cytometry in Clinical Trials. Nature Immunology 11(11), 975-978 (2010).
  12. Development of a Candidate Secondary Reference Procedure (Immunoassay Based Measurement Procedure of Higher Metrological Order) for Cardiac Troponin I: 1. Antibody Characterization and Preliminary Validation. Clin Chem Lab Med 48(11), 1603-1610 (2010).
  13. Removal of Inhibitory Effects in a Serum Cardiac Troponin I Immunoassay. Clin. Chem. 55:11, 2055-2056 (2009).
  14. Toward Quantitative Fluorescence Measurements with Multicolor Flow Cytometry. Cytometry 73A, 279-288 (2008).

NIST-NRC and NIH/NIST NRC Postdoctoral Fellowship Opportunities:

  1. Quantitative Flow Cytometry Measurements; RO#: 50.64.41.B6740
  2. Multiplexed Assays for Cell-based Production of Biopharmaceuticals; RO#: 50.64.41.B8223


Workshop 9: Control Cells or Not

Paul Wallace, Jonni S. Moore, Derek Jones, Litwin Virginia, Lili Wang, Yanli Liu
“Control Cells or Not” was an educational workshop that used surveys, lectures, and discussions to identify problems and solutions related to using commercially

Workshop 13: Building Measurement Assurance in Flow Cytometry

Lili Wang, Stephen Perfetto, Robert Hoffman, John T. Elliott, Sheng Lin-Gibson, Steven Bauer, Heba Degheidy, Judith Arcidiacono, Litwin Virginia
Two workshops were held to identify measurement challenges and potential solutions for building measurement assurance for flow cytometry. This report summarizes

Comparison of volumetric and bead-based counting of CD34 cells by single-platform flow cytometry

Luisa Saraiva, Lili Wang, Martin Kammel, A. Kummrow, E Atkinson, J Y. Lee, B Yalcinkaya, M Akgoz, A Ruf, A Engel, Yu-Zhong Zhang, O O''Shea, M P. Sassi, C Divieto, T Lekishvili, J J. Campbell, Y Liu, J Wang, R Stebbings, Adolfas Gaigalas, P Rigsby, J Neukammer, S Vessillier
Background: Over 2000 people a year in the UK need a bone marrow or blood stem cell transplant. It is important to accurately quantify the haematopoietic stem

Methodology for evaluating and comparing fluorescence measurement capabilities: Multi-site study of 23 flow cytometers

David R. Parks, Wayne A. Moore, Ryan Brinkman, Yong Chen, Danilo Condello, Faysal E. Khettabi, John P. Nolan, Stephen P. Perfetto, Doug Redelman, Josef Spidlen, Jonathan V. Dyke, Lili Wang, James C. Wood
We developed measurement methods and analysis procedures to perform inter-instrument comparisons and used them to evaluate and compare the fluorescence
Created October 9, 2019