Nathanael David Olson

Publications

Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome

August 12, 2019

Author(s):

Justin M. Zook, Nathanael D. Olson, Aaron Wenger

The DNA sequencing technologies in use today produce either highly accurate short reads or less-accurate long reads. We report the optimization of circular...

High-coverage, long-read sequencing of Chinese trio reference samples

June 14, 2019

Author(s):

Justin M. Zook, Nathanael D. Olson, Marc L. Salit, Aaron Wenger, Chunlin Xiao, Robert Sebra

Genome In a Bottle (GIAB) is a consortium hosted by the National Institute of Standards and Technology whose primary objective is the development and...

An open resource for accurately benchmarking small variant and reference calls

April 01, 2019

Author(s):

Justin M. Zook, Jennifer H. McDaniel, Marc L. Salit, Nathanael D. Olson, Justin M. Wagner

Benchmark small variant calls are required for developing, optimizing and assessing the performance of sequencing and bioinformatics methods. Here, as part of...

Impact of conventional wastewater treatment and reuse site practices on the bacterial community structure of reclaimed water

October 15, 2018

Author(s):

Prachi Kulkarni, Nathanael D. Olson, Joseph N. Paulson, Mihai Pop, Cynthia Maddox, Emma Claye, Rachel E. Rosenberg Goldstein, Manan Sharma, Amy R. Sapkota

Reclaimed water use continues to expand across the United States, from areas that have access to advanced, potable-level treated reclaimed water, to areas that...

Using metagenomic methods to detect organismal contaminants in microbial materials.

September 12, 2017

Author(s):

Nathanael D. Olson, Justin M. Zook, Jayne B. Morrow, Nancy J. Lin

High sensitivity methods as next generation sequencing and PCR are adversely impacted by organismal and DNA contaminants. Current methods for detecting...