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Hua-Jun He (Fed)

Biologist

Research Interests

  1. Standards & reference materials development for cancer biomarker measurements. Currently, we are working on the development of circulating cell-free tumor DNA (ctDNA), cell-free DNA (cfDNA) methylation, cfRNA, extracellular vesicles and other cancer biomarker reference materials for liquid biopsy. In addition, we are working on the development of next generation cell-based reference materials: a) broadly consented and characterized human tumor and matched normal cell lines for multi-omics cancer biomarker detection and benchmark testing; and b) chimeric antigen receptor-T (CAR-T) cell, CAR-NK (natural killer) and CAR engineered in other type of human cell lines for standardization of cell therapy.
  2. Metrology for cancer biomarker and biosystems. We are working on the development of digital PCR and next-generation sequencing (NGS) based robust and sensitive assays for rare allele genetic and epigenetics alterations. Other interests include adopting and validating emerging technologies for fast, sensitive, specific, and accurate cancer biomarker measurements, such as electric field induced release and measurements (EFIRM), lateral flow assays and other portable devices for liquid biopsy.

Research Opportunities

NIST-NRC Postdoctoral Research Associateship Program: 2-year fellowship at NIST, US citizens only, competitive salary plus benefits, travel stipend and relocation expenses. Application deadlines are Feb. 1 and Aug. 1. Requires a 10-page research proposal plus additional materials. Contact me if interested in writing a research proposal on cancer biomarker measurements.

Current opportunities available:

  1. Precision Measurements of Cancer Biomarkers for Liquid Biopsies
  2. Metrology for Epigenetics

Interlaboratory Testing Program

Publications

Standards and Metrology for Viral Vectors as Molecular Tools: Outcomes from a CCQM Workshop

Author(s)
Janathan Campbell, Neil Almond, Y Bae, Ravneet Bhuller, Andrea Briones, S-Y CHO, Megan Cleveland, Thomas Cleveland, Francis Galaway, Hua-Jun He, U Herbrand, Jim Huggett, Sarah Kempster, Ibolya Kepiro, Afifa Khan, Edward Kwee, Wilson Li, Sheng Lin-Gibson, Luise Luckau, Caterina Minelli, M Ryadnov, I Searing, Lili Wang, Alexandra Whale, Julian Braybrook
Viral vectors are agents enabling gene transfer and genome editing and have widespread utility across the healthcare and biotechnology sectors. In January 2023

Digital PCR for the Characterization of Reference Materials

Author(s)
Megan Cleveland, Hua-Jun He, Mojca Milavec, Young-Kyung Bae, Peter Vallone, Jim Huggett
Well characterized reference materials are essential to ensuring the harmonization and accuracy of nucleic acid-based tests (such as qPCR); digital PCR (dPCR)

Reference standards for accurate validation and optimization of assays that determine integrated Lentiviral vector copy number in transduced cells

Author(s)
Barbara S. Paugh, Lajos Baranyi, Andre Roy, Hua-Jun He, Lindsay Harris, Kenneth D. Cole, Caroline Raimund, Patricia S. Langan, Moria Artlip, Srikanta Jana, Rimas J. Orentas, Sheng Lin-Gibson, Winfried Krueger, Boro Dropuli?
Lentiviral vectors (LV) have emerged as a robust technology for therapeutic gene delivery into human cells as advanced medicinal products. As these products are

Development and Interlaboratory Evaluation of a NIST Reference Material RM 8366 for EGFR and MET Gene Copy Number Measurements

Author(s)
Hua-Jun He, Biswajit Das, Megan H. Cleveland, Chen Li, Corinne Camalier, Liang-Chu Liu, Kara L. Norman, Andrew Fellowes, Christopher McEvoy, Steven P. Lund, Jamie L. Almeida, Carolyn R. Steffen, Chris Karlovich, P. M. Williams, Kenneth D. Cole
The National Institute Standard and Technology (NIST) Reference Material RM 8366 was developed to improve the quality of gene copy measurements of EGFR
Created July 17, 2018, Updated April 4, 2023