B.S. Chemistry, Xiangtan Normal University, 1992
M.S. Analytical Chemistry, Hunan University, 1997
Ph.D. Biochemical Engineering, East China University of Science and Technology, 2000
Post Doc Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, 2000-2005
1). Standards & reference materials for cancer biomarker clinical analysis. Currently, we are working on the development of cell line based genomic DNA reference materials for gene copy number variations biomarker HER2/EGFR/MET, and spike-in based mimic cell- free DNA reference materials for cancer detection of rare allele mutation in non-invasive circulating tumor DNA (ctDNA) from patient's blood. Human artificial chromosomes (HACs) carrying a functional kinetochore were considered as a promising system for gene delivery and expression with a potential to overcome many problems caused by the use of viral-based gene transfer systems. We are interested in engineering a tailored reference material platform based on HAC and genome editing tools such as CRISPR/Cas9 for the production of defined gene copies and gene mutation, or defined fold protein overexpression reference materials.
2). Metrology for cancer biomarker and biosystems. A variety of genetic alterations, including deletions, amplifications, rearrangements and point mutations, exist in human cancer. Recently, we are working on developing digital PCR and next-generation sequencing (NGS) based robust and sensitive assays for rare allele genetic alterations. We are also interested in developing Flow-FISH for simultaneously quantitation of gene amplification and protein overexpression.