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Hua-Jun He

Research Interests

1). Standards & reference materials for cancer biomarker clinical analysis. Currently, we are working on the development of cell line based genomic DNA reference materials for gene copy number variations biomarker HER2/EGFR/MET, and spike-in based mimic cell- free DNA reference materials for cancer detection of rare allele mutation in non-invasive circulating tumor DNA (ctDNA) from patient's blood. Human artificial chromosomes (HACs) carrying a functional kinetochore were considered as a promising system for gene delivery and expression with a potential to overcome many problems caused by the use of viral-based gene transfer systems. We are interested in engineering a tailored reference material platform based on HAC and genome editing tools such as CRISPR/Cas9 for the production of defined gene copies and gene mutation, or defined fold protein overexpression reference materials.

2). Metrology for cancer biomarker and biosystems. A variety of genetic alterations, including deletions, amplifications, rearrangements and point mutations, exist in human cancer. Recently, we are working on developing digital PCR and next-generation sequencing (NGS) based robust and sensitive assays for rare allele genetic alterations. We are also interested in developing Flow-FISH for simultaneously quantitation of gene amplification and protein overexpression.

 

NRC POSTDOCTORAL FELLOWSHIP OPPORTUNITIES:

http://nrc58.nas.edu/RAPLab10/Opportunity/Opportunity.aspx?LabCode=50&ROPCD=506441&RONum=C0094
http://nrc58.nas.edu/RAPLab10/Opportunity/Opportunity.aspx?LabCode=50&ROPCD=506441&RONum=B8574

 

 

 

Publications

Development and Interlaboratory Evaluation of a NIST Reference Material RM 8366 for EGFR and MET Gene Copy Number Measurements

Author(s)
Hua-Jun He, Biswajit Das, Megan H. Cleveland, Chen Li, Corinne Camalier, Liang-Chu Liu, Kara L. Norman, Andrew Fellowes, Christopher McEvoy, Steven P. Lund, Jamie L. Almeida, Carolyn R. Steffen, Chris Karlovich, P. M. Williams, Kenneth D. Cole
The National Institute Standard and Technology (NIST) Reference Material RM 8366 was developed to improve the quality of gene copy measurements of EGFR

Multi-Laboratory Assessment of a New Reference Material for Quality Assurance of Cell-Free Tumor DNA Measurements

Author(s)
Hua-Jun He, Erica V. Stein, Yves Konigshofer, Thomas Forbes, Farol L. Tomson, Russell Garlick, Emiko Yamada, Tony Godfrey, Toshiya Abe, Koji Tamura, Michael Borges, Michael Goggins, Sandra Elmore, Margaret L. Gulley, Jessica L. Larson, Lando Ringel, Brian C. Haynes, Corinne Camalier, Chris Karlovich, Biswajit Das, P. M. Williams, Aaron Garnett, Anders Stahlberg, Stefan Filges, Lynn Sorbara, Mathew R. Young, Sudhir Srivastava, Kenneth D. Cole
We conducted a multi-laboratory assessment to determine the suitability of a new commercially- available reference material with 40 cancer variants in a

Certified DNA Reference Materials to Compare HER2 Gene Amplification Measurements Using Next- Generation Sequencing Methods

Author(s)
Chih-Jian Lih, Robin D. Harrington, Kneshay Harper, David J. Sims, Paul McGregor, Corinne Camalier, Han Si, Biswajit Das, P. M. Williams, Hua-Jun He, Jamie L. Almeida, Steven P. Lund, Steven J. Choquette, Kenneth D. Cole
The National Institute of Standards and Technology (NIST) Standard Reference Materials 2373 is a set of genomic DNA samples prepared from five breast cancer
Created July 17, 2018, Updated May 16, 2019