Protocols Supporting Bioeconomy Standards

Laboratory Automation for Engineering Biology

• Method for reproducible automated bacterial cell culture and measurement, Synthetic Biology, Volume 7, Issue 1, 2022, ysac013 - NIST developed this protocol to enable better quantitative measurements of engineered microbes through reproducible microbial cell culture and measurement using state-of-the-art automation.
• Protocols for automated DNA extraction from microbial cells
  • Automation Protocol for Plasmid DNA Extraction from E. coli
  • Automation Protocol for High-Efficiency and High-Quality Genomic DNA Extraction from Saccharomyces Cerevisiae V.4  as published in PLOS One

Large-Scale Sequence–Function Datasets

•  Protein Sequence to Function, Align to Innovate Dataset - NIST is collaborating with Align to Innovate and other institutions to develop standardized methods for the collection, analysis, and reporting of large-scale, quantitative protein sequence–function datasets that will span multiple function types.
• Protocols for quantitative calibration of large-scale sequence–function datasets
  • Singleplex Assay for Function Measurements
  • Singleplex Assay for Fitness Measurements
• Protocols for collection of large-scale sequence–function datasets
  • Pooled, Growth-Based Assays
  • Library Bottlenecking Protocols
  • Automated Bar-Seq Library Preparation and Pooling

Cell-Free Systems

• Automated DNA template preparation and quantitation methods
• Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production as published in Cell-Free Gene Expression