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Search Publications by: Carl G. Simon, Jr., Ph.D. (Fed)

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Displaying 51 - 75 of 132

Osteoblast Response to Serum Protein Adsorption in 3D Polymer Scaffolds

May 1, 2012
Author(s)
Carl G. Simon Jr., Girish Kumar, Kaushik Chatterjee, Stevephen Hung
Protein adsorption is known to direct biological response to biomaterials and is important in determining cellular response in tissue scaffolds. In this study we investigated the effect of adsorbed serum proteins on cell attachment and proliferation in

Three-dimensional Shape Metrology for Tissue Engineering

April 21, 2012
Author(s)
Joy P. Dunkers, Antonio M. Possolo, Thomas V. Lafarge, Beatriz Pateiro-Lopez, Jeffrey Coles, Carl G. Simon Jr.
The physical and chemical properties of a tissue scaffold influences cell response and therefore the success of the resulting tissue construct. Scaffold structure from nanometers to millimeters can direct cell behavior, from viability to proliferation to

Freeform Fabricated Scaffolds with Roughened Struts that Enhance both Stem Cell Proliferation and Differentiation by Controlling Cell Shape

March 1, 2012
Author(s)
Girish Kumar, Carl G. Simon Jr., Michael S. Waters, Tanya M. Farooque, Marian F. Young
We demonstrate that imparting freeform fabricated (FFF) scaffolds with surface roughness on their struts enhances osteogenic differentiation of primary human bone marrow stromal cells (hBMSCs) by controlling cell shape. Previous work showed that hBMSCs

Morphological Changes Driven by Nanofibrous Scaffolds Induce Marrow Stromal Cell Osteogenesis

October 11, 2011
Author(s)
Carl G. Simon Jr., Kaushik Chatterjee, Christopher K. Tison, Girish Kumar, Patrick S. Pine, Marc L. Salit, Jennifer H. McDaniel, Marian F. Young
Cells are sensitive to tissue scaffold architecture and these cell-material interactions drive cell functions critical in tissue regeneration. Results presented here demonstrate that nanofiber scaffolds force primary human bone marrow stromal cells (hBMSCs

Cell Adhesion to Borate Glasses by Colloidal Probe Microscopy

August 1, 2011
Author(s)
Sheldon M. Wiederhorn, Carl G. Simon Jr., Young-Hun Chae
The adhesion of Osteoblast-like cells to silicate and borate glasses was measured using colloidal probe microscopy in a cell growth medium. The probe consisted of silicate and borate glass spheres, 25 µm to 50 µm in diameter, attached to the tip of an

Combinatorial and High-throughput Screening of Biomaterials

June 6, 2011
Author(s)
Carl Simon Jr., Sheng Lin-Gibson
The review describes recent advances in utilizing combinatorial and high-throughput methods to better understand cell-material interactions, in terms of cell toxicity, adhesion, morphology, migration, proliferation and differentiation, particularly

Imaging Cells in Polymer Scaffolds by X-Ray Microcomputed Tomography

May 11, 2011
Author(s)
Shauna M. Dorsey, Sheng Lin-Gibson, Carl Simon Jr.
We have investigated the ability of X-ray microcomputed tomography (CT) to make quantitative, three-dimensional (3D) measurements of cell adhesion and proliferation in polymeric tissue engineering scaffolds. The most common method for examining cells in

Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture

May 2, 2011
Author(s)
Kaushik Chatterjee, Marian F. Young, Carl Simon Jr.
Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties

NIST Reference Material Scaffolds Characterized for Cell Response

April 24, 2011
Author(s)
Carl G. Simon Jr., Jeff M. Coles
Reference scaffolds characterized for cell response are being developed for use as a standard for biological characterization of new scaffolds. The need for reference scaffolds to serve as a calibration standard between labs has been identified as critical

Development of Probe Free Mapping of Cell Viability in Hydrogels

April 16, 2011
Author(s)
Joy P. Dunkers, Kaushik Chatterjee, Carl G. Simon Jr.
Diffusion of oxygen in cell/scaffold construct interior is of critical importance to cell viability in constructs of clinically relevant sizes. It has been shown that depletion of oxygen within the scaffold leads to a decrease in cell density and viability

Modulus-Driven Differentiation of Marrow Stromal Cells in 3D Is Independent of Cytoskeletal Integrity

April 11, 2011
Author(s)
Carl G. Simon Jr., Kaushik Chatterjee, Sapun Parekh, Sheng Lin-Gibson, Nicole M. Moore, Marcus T. Cicerone, Marian F. Young
Cell functions such as proliferation, migration, and differentiation are key physiological processes that are influenced by the physiochemical extracellular environment. We report on the effect of three-dimensional (3D) scaffold modulus on human bone

3D Polymer Scaffold Arrays

December 5, 2010
Author(s)
Carl G. Simon Jr., Yanyin Yang, Shauna M. Dorsey, Murugan Ramalingam, Kaushik Chatterjee
We have developed a combinatorial platform for screening the effect polymer scaffold properties and composition on cell response. Traditional research involves preparing samples one at a time for characterization and testing. Combinatorial and high

Effect of 3D Hydrogel Scaffold Stiffness on Human Bone Marrow Stromal Cell Differentiation

November 1, 2010
Author(s)
Carl G. Simon Jr., Sapun Parekh, Marcus T. Cicerone, Sheng Lin-Gibson, Kaushik Chatterjee, Marian F. Young
There is growing recognition cells can sense and respond to the mechanical properties of tissue scaffolds and that these interactions are critical in optimizing scaffold design [1-4]. Previous studies in planar 2D culture format have shown that human bone

Effect of 3D Hydogel Scaffold Modulus and Topology on Human Bone Marrow Stromal Cell Fate

September 1, 2010
Author(s)
Kaushik Chatterjee, Sheng Lin-Gibson, Sapun Parekh, Nicole M. Moore, Marcus T. Cicerone, marian F. young, Carl Simon Jr.
There is growing recognition that cells can sense and respond to the physical cues from their environment, such as stiffness, mechanical loading and topology. Physical properties of the matrix can direct cellular response and are critical in the design of

Gradient Nanofiber Scaffold Libraries for Rapid Screening of Cell-Material Interactions

December 1, 2009
Author(s)
Carl G. Simon Jr., Murugan Ramalingam, Marian F. Young, Vinoy Thomas
Scaffolds play a key role in tissue engineering wherein they provide structural support for cells to adhere, grow and guide them to synthesize tissue. Scaffolds made of multiple biomaterials are typically required to mimic the three-dimensional (3D)

NIST Reference Material Scaffolds for Tissue Engineering

December 1, 2009
Author(s)
Carl G. Simon Jr.
The National Institute of Standards and Technology (NIST) has deployed Reference Material (RM) scaffolds for tissue engineering: a series of well-characterized 3D tissue scaffolds with differing porosities (RM 8395, RM 8396 and RM 8397) (Fig. 1). Customers