Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture
Kaushik Chatterjee, Marian F. Young, Carl Simon Jr.
Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We describe a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we focus on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.