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Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture

Published

Author(s)

Kaushik Chatterjee, Marian F. Young, Carl Simon Jr.

Abstract

Optimizing cell-material interactions is critical towards maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We describe a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we focus on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.
Citation
Methods In Bioengineering
Publisher Info
Artech House, Norwood, MA

Keywords

combinatorial methods, high-throughput screening, hydrogels, elastic modulus, gradients, osteoblast, tissue engineering

Citation

Chatterjee, K. , Young, M. and Simon Jr., C. (2011), Combinatorial Screening of Hydrogel Scaffolds for 3D Cell Culture, Artech House, Norwood, MA (Accessed April 20, 2024)
Created May 1, 2011, Updated October 12, 2021