NIST Reference Material Scaffolds Characterized for Cell Response

Published

April 24, 2011

Author(s)

Carl Simon, Jeff M. Coles

Abstract

Reference scaffolds characterized for cell response are being developed for use as a standard for biological characterization of new scaffolds. The need for reference scaffolds to serve as a calibration standard between labs has been identified as critical to advancing tissue engineering science [1,2]. The Regenerative Medicine Promotion Act was recently introduced into the U.S. House of Representatives which specifically calls for NIST to develop standards for regenerative medicine products [3]. Previously, NIST deployed reference scaffolds with well-characterized structure and porosity (Fig. 1) [4]. The current effort expands the scope to include cell culture data for morphology, attachment and proliferation. Freeform fabrication (FFF) was chosen to make the reference scaffolds since this technique affords precise control of scaffold structure. Poly(-caprolactone) (PCL) was chosen as the polymer since it is used in biomedical implants and is being investigated for tissue scaffold applications. MC3T3-E1 osteoblasts were used for cell response since scaffolds are frequently used for bone tissue engineering. 96-well plate scaffolds were selected for efficiency (fewer reagents, lower cost).

Conference Dates

April 13-16, 2011

Conference Location

Orlando, FL, US

Conference Title

Society for Biomaterials

Pub Type

Conferences

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Keywords

reference material, scaffold, polymer, tissue engineering, cell proliferation, cell adhesion, freeform fabricated scaffold

Citation

Simon, C. and Coles, J. (2011), NIST Reference Material Scaffolds Characterized for Cell Response, Society for Biomaterials, Orlando, FL, US, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=907327 (Accessed May 19, 2026)

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