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NIST Reference Material Scaffolds Characterized for Cell Response



Carl G. Simon Jr., Jeff M. Coles


Reference scaffolds characterized for cell response are being developed for use as a standard for biological characterization of new scaffolds. The need for reference scaffolds to serve as a calibration standard between labs has been identified as critical to advancing tissue engineering science [1,2]. The Regenerative Medicine Promotion Act was recently introduced into the U.S. House of Representatives which specifically calls for NIST to develop standards for regenerative medicine products [3]. Previously, NIST deployed reference scaffolds with well-characterized structure and porosity (Fig. 1) [4]. The current effort expands the scope to include cell culture data for morphology, attachment and proliferation. Freeform fabrication (FFF) was chosen to make the reference scaffolds since this technique affords precise control of scaffold structure. Poly(-caprolactone) (PCL) was chosen as the polymer since it is used in biomedical implants and is being investigated for tissue scaffold applications. MC3T3-E1 osteoblasts were used for cell response since scaffolds are frequently used for bone tissue engineering. 96-well plate scaffolds were selected for efficiency (fewer reagents, lower cost).
Conference Dates
April 13-16, 2011
Conference Location
Orlando, FL
Conference Title
Society for Biomaterials


reference material, scaffold, polymer, tissue engineering, cell proliferation, cell adhesion, freeform fabricated scaffold


Simon, C. and Coles, J. (2011), NIST Reference Material Scaffolds Characterized for Cell Response, Society for Biomaterials, Orlando, FL (Accessed July 16, 2024)


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Created April 24, 2011, Updated February 19, 2017