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Development of Probe Free Mapping of Cell Viability in Hydrogels



Joy P. Dunkers, Kaushik Chatterjee, Carl G. Simon Jr.


Diffusion of oxygen in cell/scaffold construct interior is of critical importance to cell viability in constructs of clinically relevant sizes. It has been shown that depletion of oxygen within the scaffold leads to a decrease in cell density and viability. Non-destructive methods that use fluorophore labeling to assess cell viability are also susceptible to problems with probe diffusion to the scaffold interior. Here we seek to develop a method for in vitro, spatial mapping of cell viability without using fluorescent labeling. The goal of this work is to establish contrast mechanisms for distinguishing between live and dead cells using a high sensitivity technique called optical coherence microscopy.
Proceedings Title
2011 Society for Biomaterials Annual Meeting
Conference Dates
April 13-16, 2011
Conference Location
Orlando, FL


optical coherence microscopy, hydrogel, imaging, viability


Dunkers, J. , Chatterjee, K. and Simon, C. (2011), Development of Probe Free Mapping of Cell Viability in Hydrogels, 2011 Society for Biomaterials Annual Meeting , Orlando, FL, [online], (Accessed April 23, 2024)
Created April 16, 2011, Updated February 19, 2017