Development of Probe Free Mapping of Cell Viability in Hydrogels

Published

April 16, 2011

Author(s)

Joy P. Dunkers, Kaushik Chatterjee, Carl G. Simon Jr.

Abstract

Diffusion of oxygen in cell/scaffold construct interior is of critical importance to cell viability in constructs of clinically relevant sizes. It has been shown that depletion of oxygen within the scaffold leads to a decrease in cell density and viability. Non-destructive methods that use fluorophore labeling to assess cell viability are also susceptible to problems with probe diffusion to the scaffold interior. Here we seek to develop a method for in vitro, spatial mapping of cell viability without using fluorescent labeling. The goal of this work is to establish contrast mechanisms for distinguishing between live and dead cells using a high sensitivity technique called optical coherence microscopy.

Proceedings Title

2011 Society for Biomaterials Annual Meeting

Volume

1

Issue

1

Conference Dates

April 13-16, 2011

Conference Location

Orlando, FL

Pub Type

Conferences

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Keywords

optical coherence microscopy, hydrogel, imaging, viability

Citation

Dunkers, J. , Chatterjee, K. and Simon, C. (2011), Development of Probe Free Mapping of Cell Viability in Hydrogels, 2011 Society for Biomaterials Annual Meeting , Orlando, FL, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=907336 (Accessed October 23, 2025)