Combinatorial Screening of Osteoblast Response to 3D Nano-Composite Tissue Scaffolds Using Gradients and Arrays
Carl G. Simon Jr., Kaushik Chatterjee, Limin Sun, Laurence C. Chow, Marian F. Young
Current methods for combinatorial and high-throughput (CHT) screening of cell-material interactions utilize a two-dimensional (2D) format where cells are presented on flat materials surfaces . However, cell response in 3D scaffolds is more representative of their behavior in vivo . Also, biomaterials must be fabricated into a 3D scaffold to generate a 3D tissue and cells are sensitive to topographical differences between 2D surfaces and 3D scaffolds. Thus, the objective in this work was to develop CHT methods to screen cell-material interactions in 3D. We used a bone tissue model to study osteoblast attachment and proliferation on porous poly(-caprolactone) (PCL) scaffolds containing amorphous calcium phosphate nanoparticles (nACP). nACP is highly soluble in water and releases calcium and phosphate ions that are known to enhance osteoblast response.
, Chatterjee, K.
, Sun, L.
, Chow, L.
and Young, M.
Combinatorial Screening of Osteoblast Response to 3D Nano-Composite Tissue Scaffolds Using Gradients and Arrays, Society for Biomaterials, Orlando, FL
(Accessed June 9, 2023)