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Combinatorial Screening of Osteoblast Response to 3D Nano-Composite Tissue Scaffolds Using Gradients and Arrays

Published

Author(s)

Carl G. Simon Jr., Kaushik Chatterjee, Limin Sun, Laurence C. Chow, Marian F. Young

Abstract

Current methods for combinatorial and high-throughput (CHT) screening of cell-material interactions utilize a two-dimensional (2D) format where cells are presented on flat materials surfaces [1]. However, cell response in 3D scaffolds is more representative of their behavior in vivo [2]. Also, biomaterials must be fabricated into a 3D scaffold to generate a 3D tissue and cells are sensitive to topographical differences between 2D surfaces and 3D scaffolds. Thus, the objective in this work was to develop CHT methods to screen cell-material interactions in 3D. We used a bone tissue model to study osteoblast attachment and proliferation on porous poly(-caprolactone) (PCL) scaffolds containing amorphous calcium phosphate nanoparticles (nACP). nACP is highly soluble in water and releases calcium and phosphate ions that are known to enhance osteoblast response.
Conference Dates
April 13-16, 2011
Conference Location
Orlando, FL
Conference Title
Society for Biomaterials

Keywords

polymer scaffold, osteoblast, cell adhesion, cell proliferation

Citation

Simon, C. , Chatterjee, K. , Sun, L. , Chow, L. and Young, M. (2011), Combinatorial Screening of Osteoblast Response to 3D Nano-Composite Tissue Scaffolds Using Gradients and Arrays, Society for Biomaterials, Orlando, FL (Accessed April 18, 2024)
Created April 24, 2011, Updated February 19, 2017