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Search Publications by: Michael J. Tarlov (Fed)

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Displaying 26 - 50 of 61

DNA Displacement Assay Integrated into Microfluidic Channels

July 1, 2004
Author(s)
Rebecca A. Zangmeister, Michael J. Tarlov
This paper describes the development of a unique fluorescence based DNA diagnostic assay incorporated into a microfluidic channel that does not require labeling of the target sequence prior to analysis. The assay is based on the displacement of a short

Atmospheric Pressure Microplasmas for Modifying Sealed Microfluidic Devices

March 8, 2004
Author(s)
J K. Evju, P B. Howell, Laurie E. Locascio, Michael J. Tarlov, J J. Hickman
A new DC microdischarge technique for chemical modification of microchannel walls is reported. Our results show that strongly hydrophilic or hydrophobic properties are quickly imparted unto the walls of assembled polymer microchannels in atmospheric

Chiral Separations by Temperature Gradient Focusing

February 1, 2004
Author(s)
K M. Balss, Wyatt N. Vreeland, Karen W. Phinney, Michael J. Tarlov, David J. Ross
Temperature gradient focusing (TGF) involves application of a temperature gradient across a microchannel or capillary. With an appropriate buffer, the temperature gradient creates a gradient in both the electric field and the electrophoretic velocity

Quantitative Characterization of DNA Films by X-ray photoelectron spectroscopy

January 20, 2004
Author(s)
D Y. Petrovykh, H Y. Kimura-Suda, Michael J. Tarlov, L J. Whitman
Self-assembled films of thiolated poly(dT)25 single-stranded DNA (ssDNA) on gold areintroduced as a model system for quantitative characterization of DNA films by XPS. Weevaluate the applicability of a simple substrate-overlayer model for data analysis

Selective DNA Sensing Elements Integrated Into Microfluidic Channels

December 1, 2003
Author(s)
Rebecca A. Zangmeister, Michael J. Tarlov
This paper describes the development of a unique fluorescence based DNA diagnostic assay that does not require pre-labeling of the target oligomer prior to analysis. The assay uses polyacrylamide hydrogel plugs immobilized in microfluidic channels, and is

Base-Dependent Competitive Adsorption of Single-Stranded DNA on Gold

July 30, 2003
Author(s)
H Y. Kimura-Suda, D Y. Petrovykh, Michael J. Tarlov, L J. Whitman
Aqueous solutions of single-stranded DNA probes (ssDNA) are widely used with gold surfaces in current biotechnology and emerging nanotechnology applications. Although recent studies have shown that different DNA bases interact differentlywith gold surfaces

Effect of Surface Wettability on Fast Transient Microboiling Behavior

July 22, 2003
Author(s)
O C. Thomas, Richard E. Cavicchi, Michael J. Tarlov
Microboiling events associated with the fast transient heating of a micrometer-scale metallic thin film heater immersed in water have been studied. The effect of surface properties on the microboiling transients was examined by modifying heater surfaces

Quantitative Analysis and Characterization of DNA Immobilized on Gold

April 30, 2003
Author(s)
D Y. Petrovykh, H Y. Kimura-Suda, L J. Whitman, Michael J. Tarlov
We describe the complementary use of x-ray photoelectron spectroscopy (XPS) and fourier transform infrared (FTIR) spectroscopy to quantitatively characterize the immobilization of thiolated poly(dT)25 single-stranded DNA (ssDNA) on gold. When electron

DNA-Based Sensors

January 1, 2003
Author(s)
Michael J. Tarlov, A B. Steel
The development of DNA-based sensors for genetic assay and sequencing applications has exploded over the last decade. Nearly all of these devices rely on the hybridization of a complementary oligonucleotide with surface-confined, single-stranded DNA or RNA

Immobilization of DNA Hydrogel Plugs in Microfluidic Channels

March 15, 2002
Author(s)
K G. Olsen, David J. Ross, Michael J. Tarlov
Acrylamide-modified DNA probes are immobilized in polycarbonate microfluidic channels via photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions and hybridize with fluorescently

DNA Hydrogel Plugs Immobilized in Microfluidic Channels with Improved Stability

February 28, 2002
Author(s)
Rebecca A. Zangmeister, K G. Olsen, David J. Ross, Michael J. Tarlov
It has been previously demonstrated that DNA, modified on the 5' end with an acrylic acid group, can be incorporated into a polyacrylamide hydrogel matrix. These types of DNA containing gels have recently been immobilized in plastic microfluidic channels

Surface Characterization of Laser- Ablated Polymers Used for Microfluidics

February 15, 2002
Author(s)
D L. Pugmire, E A. Waddell, R Haasch, Michael J. Tarlov, Laurie E. Locascio
Fabrication of microfluidic devices by excimer laser ablation under different atmospheres may provide variations in polymer microchannel surface characteristics. The surface chemistry and electroosmotic (EO) mobility of polymer microchannels laser ablated

Sol-Gel Materials for Gas Phase Sensing Using Microhotplate Arrays

February 1, 2002
Author(s)
N O. Savage, Richard E. Cavicchi, Michael J. Tarlov, Stephen Semancik, J Greg Gillen
Sol-gel chemistry is used to create suspensions (sols) of small particles of materials such as metal oxides. These suspensions can be dried to a gel or powder or used in the sol form for coatings and thin films. One promising application of sol-gel derived

Fabrication, Derivatization and Applications of Plastic Microfluidic Devices

February 1, 2001
Author(s)
S L. Barker, Michael J. Tarlov, David J. Ross, T J. Johnson, E A. Waddell, Laurie E. Locascio
Control of the polymer surface chemistry is a crucial aspect of development of plastic microfluidic devices. When commercially available plastic substrates are used to fabricate microchannels, differences in the electroosmotic flow mobility (EOF) from

A Surface Chemistry Study of Laser Ablated Plastics Used for Microfluidic Devices

January 1, 2001
Author(s)
D P. Pugmire, E A. Waddell, Michael J. Tarlov
Plastic substrates are being investigated for use in microfluidic devices because of their low cost, ease of fabrication, and wide range of materials properties. It is well established that the surface chemistry of a plastic substrate greatly influences

Control of Flow Direction in Microfluidic Devices with Polyelectrolyte Multilayers

December 1, 2000
Author(s)
S L. Barker, David J. Ross, Michael J. Tarlov, Michael Gaitan, Laurie E. Locascio
Electroosmotic flow (EOF) is commonly utilized in microfluidics. Because the direction of the EOF can be determined by the substrate surface charge, control of the surface chemical state offers the potential, in addition to voltage control, to direct the

Plastic Microfluidic Devices Modified With Polyelectrolyte Multilayers

October 15, 2000
Author(s)
S L. Barker, Michael J. Tarlov, H E. Canavan, J J. Hickman, Laurie E. Locascio
Control of the polymer surface chemistry is a crucial aspect of development of plastic microfluidic devices. When commercially available plastic substrates are used to fabricate microchannels, differences in the EOF mobility from plastic to plastic can be

Derivatization of Plastic Microfluidic Devices With Polyelectrolyte Multilayers

May 1, 2000
Author(s)
S L. Barker, Michael J. Tarlov, M L. Branham, J Xu, William A. MacCrehan, Michael Gaitan, Laurie E. Locascio
Microchannels fabricated in plastic materials by room temperature imprinting demonstrate large variability in surface charge as a result of the fabrication procedure. Surface charged groups are primarily localized on the channel walls and not on the