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Selective DNA Sensing Elements Integrated Into Microfluidic Channels

Published

Author(s)

Rebecca A. Zangmeister, Michael J. Tarlov

Abstract

This paper describes the development of a unique fluorescence based DNA diagnostic assay that does not require pre-labeling of the target oligomer prior to analysis. The assay uses polyacrylamide hydrogel plugs immobilized in microfluidic channels, and is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence of interest as it is electrophoresed through the hydrogel plug sensing platform. The distinct advantages of this assay are the short sensing times as a result of directed electrophoretic transport of target DNA to the sensing element and the ability to detect non-labeled target DNA.
Proceedings Title
Proceedings of uTAS 2003 Seventh International Conference on Micro Total Analysis Systems
Volume
2
Conference Dates
October 5-9, 2003
Conference Location
Squaw Valley, CA
Conference Title
MicroTAS 2003 Conference - Seventh International Conference on Micro Total Analysis Systems

Keywords

Displacement assay, DNA sensing, Microfluidics, Polyacrylamide hydrogel

Citation

Zangmeister, R. and Tarlov, M. (2003), Selective DNA Sensing Elements Integrated Into Microfluidic Channels, Proceedings of uTAS 2003 Seventh International Conference on Micro Total Analysis Systems, Squaw Valley, CA (Accessed June 13, 2024)

Issues

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Created December 1, 2003, Updated February 19, 2017