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Temperature Gradient Focusing of Matched and Partially Mismatched DNA/PNA Hybridizations

Published

Author(s)

K M. Balss, David J. Ross, Michael J. Tarlov

Abstract

The hybridization reactions of DNA with peptide nucleic acids (PNA) were monitored in real time with Temperature Gradient Focusing (TGF). Both ex situ and in situ reactions of a perfect complement and mismatched ssDNA targets with PNA were monitored by fluorescence microscopy. TGF was used to first spatially focus a plug of ssDNA within a microchannel and then to hold the plug stationary for hybridization with a peptide nucleic acid probe transported by bulk flow of the buffer. The mismatched ssDNA target did not react with the PNA. TGF of PNA/DNA demonstrated a 500-fold increase in concentration during a 20 minute experiment.
Citation
International Conference on Miniaturized Chemical and BioChemical Analysis Systems
Volume
126
Issue
41

Keywords

concentration method, DNA hybridizations, peptide nucleic acid (PNA), separations

Citation

Balss, K. , Ross, D. and Tarlov, M. (2004), Temperature Gradient Focusing of Matched and Partially Mismatched DNA/PNA Hybridizations, International Conference on Miniaturized Chemical and BioChemical Analysis Systems (Accessed April 15, 2024)
Created October 20, 2004, Updated February 17, 2017