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DNA Displacement Assay Integrated into Microfluidic Channels

Published

Author(s)

Rebecca A. Zangmeister, Michael J. Tarlov

Abstract

This paper describes the development of a unique fluorescence based DNA diagnostic assay incorporated into a microfluidic channel that does not require labeling of the target sequence prior to analysis. The assay is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence as it is electrophoresed through a hydrogel plug sensing platform immobilized in microfluidic channels. The distinct advantages of this assay are the short sensing times, as a result of directed electrophoretic transport of target DNA to the sensing element, combined with the ability to detect non-labeled target DNA.
Citation
Analytical Chemistry
Volume
76
Issue
No. 13

Keywords

Displacement assay, DNA sensing, Microfluidics, Polyacrylamide hydrogel

Citation

Zangmeister, R. and Tarlov, M. (2004), DNA Displacement Assay Integrated into Microfluidic Channels, Analytical Chemistry (Accessed April 14, 2024)
Created July 1, 2004, Updated February 17, 2017