Zangmeister, R.
and Tarlov, M.
(2004),
DNA Displacement Assay Integrated into Microfluidic Channels, Analytical Chemistry
(Accessed December 2, 2024)
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
DNA Displacement Assay Integrated into Microfluidic Channels
Published
Author(s)
,
Abstract
This paper describes the development of a unique fluorescence based DNA diagnostic assay incorporated into a microfluidic channel that does not require labeling of the target sequence prior to analysis. The assay is based on the displacement of a short sacrificial fluorescent-tagged indicator oligomer by a longer untagged target sequence as it is electrophoresed through a hydrogel plug sensing platform immobilized in microfluidic channels. The distinct advantages of this assay are the short sensing times, as a result of directed electrophoretic transport of target DNA to the sensing element, combined with the ability to detect non-labeled target DNA.Citation
Analytical Chemistry
Volume
76
Issue
No. 13
Pub Type
Journals
Keywords
Displacement assay, DNA sensing, Microfluidics, Polyacrylamide hydrogel
Citation
Issues
If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.
Created July 1, 2004, Updated February 17, 2017