Surface Immobilized DNA Probes on Silicon Microhotplates and Their Application in DNAAnalysis

Published

January 1, 2001

Author(s)

T H. Huang, Richard E. Cavicchi, S L. Barker, Stephen Semancik, Michael J. Tarlov

Abstract

The use of silicon machined microhotplate devices to study DNA hybridization andmelting reactions is demonstrated. The microhotplates serve both as the surfacefor immobilizing DNA probes and a heating source for study of thermal transitions of double stranded DNA. Two immobilization schemes are described for attaching DNA probes to the surface of the microhotplates. In the first system, the DNA probes are attached to the microhotplate using a method developed for forming thermally stable polyacrylamide copolymers that contains the DNA probes. The second system utilizes the self-assembly of thiolated ssDNA on gold for surface attachment of probes. DNA hybridization and melting events are monitored using fluorescence microscopy/spectroscopy. Parameters that can influence the ability of the anchored probes to monitor DNA melting and hybridization are investigated.

Citation

Surface Immobilized DNA Probes on Silicon Microhotplates and Their Application in DNAAnalysis

Pub Type

Others

Keywords

DNA, DNA hybridization, DNA melting, DNA probes, microhotplate devices, silicon heated microhotplate devices

Citation

Huang, T. , Cavicchi, R. , Barker, S. , Semancik, S. and Tarlov, M. (2001), Surface Immobilized DNA Probes on Silicon Microhotplates and Their Application in DNAAnalysis, Surface Immobilized DNA Probes on Silicon Microhotplates and Their Application in DNAAnalysis (Accessed December 13, 2024)