Olsen, K.
, Ross, D.
and Tarlov, M.
(2002),
Immobilization of DNA Hydrogel Plugs in Microfluidic Channels, Analytical Chemistry
(Accessed April 26, 2024)
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Immobilization of DNA Hydrogel Plugs in Microfluidic Channels
Published
Author(s)
K G. Olsen, ,
Abstract
Acrylamide-modified DNA probes are immobilized in polycarbonate microfluidic channels via photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions and hybridize with fluorescently-tagged complementary DNA. The double stranded DNA can be chemically denatured and the chip may be reused with a new analytical sample. Conditions for photopolymerization, hybridization, and denaturation are discussed. We also demonstrate the photopolymerization of plugs containing different DNA probe sequences in one microfluidic channel, thereby enabling the selective detection of multiple DNA targets in one electrophoretic pathway.Citation
Analytical Chemistry
Volume
74
Issue
No. 6
Pub Type
Journals
Keywords
DNA, DNA copolymer, electrophoresis, gel plug, hybridization, hydrogel, microchannel, microfluidics, polyacrylamide
Citation
Created March 14, 2002, Updated October 12, 2021