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Immobilization of DNA Hydrogel Plugs in Microfluidic Channels



K G. Olsen, David J. Ross, Michael J. Tarlov


Acrylamide-modified DNA probes are immobilized in polycarbonate microfluidic channels via photopolymerization in a polyacrylamide matrix. The resulting polymeric, hydrogel plugs are porous under electrophoretic conditions and hybridize with fluorescently-tagged complementary DNA. The double stranded DNA can be chemically denatured and the chip may be reused with a new analytical sample. Conditions for photopolymerization, hybridization, and denaturation are discussed. We also demonstrate the photopolymerization of plugs containing different DNA probe sequences in one microfluidic channel, thereby enabling the selective detection of multiple DNA targets in one electrophoretic pathway.
Analytical Chemistry
No. 6


DNA, DNA copolymer, electrophoresis, gel plug, hybridization, hydrogel, microchannel, microfluidics, polyacrylamide


Olsen, K. , Ross, D. and Tarlov, M. (2002), Immobilization of DNA Hydrogel Plugs in Microfluidic Channels, Analytical Chemistry (Accessed July 14, 2024)


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Created March 14, 2002, Updated October 12, 2021