Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Reference Scaffolds

NOTE: The NIST reference scaffolds have been discontinued due to lack of use. This page is an archive and is no longer current. 

Reference Material Scaffolds: Reference material scaffolds have been developed to enhance comparability of scaffold measurements made in different labs.


How Do You Use Reference Scaffolds? If two different labs run the NIST reference scaffolds as a control in their tests, then scaffold measurements from different labs can be compared. The y-axis may be a scaffold measurement such as cell adhesion.

Links to Reference Scaffolds:  RM8394 Scaffolds for Cell Culture

These are freeform fabricated scaffolds that fit into a 96-well plate for cell culture experiments. A unit is 24 scaffolds that have been structurally characterized and for which the cell responses of cell adhesion and proliferation have been measured. The reference scaffold plate can be run as a control in cell culture experiments to serve as a comparison point between different labs.


Left: Reference material scaffolds RM8394 for cell culture. One unit is a 96-well plate with 24 scaffolds. RM8394 has nominal strut diameter 300 μm, nominal strut spacing 500 μm and porosity 60%. Middle: 3D X-ray tomograph of RM8394. Right: Fluorescence micrograph of MC3T3-E1 osteoblasts cultured 1 d on RM8394. Red staining is actin and green staining is the nucleus.

Contact: Carl Simon


Biomaterials Group

Created May 24, 2016, Updated July 9, 2021