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Bioscience

Cell measurements

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Displaying 26 - 50 of 79

Characteristics to Consider when Selecting a Positive Control Material for an In Vitro Assay

September 29, 2021
Author(s)
Elijah Petersen, Andrew Nguyen, Jeffrey Brown, John T. Elliott, Amy Clippinger, John Gordon, Nicole Kleinstreuer, Matthias Roesslein
The use of in vitro assays to inform decision-making requires robust and reproducible results across studies, laboratories, and time. Experiments using positive control materials are an integral component of an assay procedure to demonstrate the extent to

Resources for developing reliable and reproducible in vitro test methods

September 29, 2021
Author(s)
Elijah Petersen, Vytas Reipa, Menghang Xia, Monita Sharma
A broad range of in vitro test methods have been developed given their numerous potential advantages over in vivo tests. We describe here key resources and tools to increase the reliability and reproducibility of in vitro test methods.

Use of cause-and-effect analysis to optimize the reliability of in vitro inhalation toxicity measurements using an air-liquid interface

September 29, 2021
Author(s)
Elijah Petersen, Monita Sharma, Amy Clippinger, John Gordon, Aaron Katz, Peter Laux, Lars Leibrock, Andreas Luch, Joanna Matheson, Andreas Stucki, Jutta Tentschert, Frank Bierkandt
In vitro inhalation toxicology methods are increasingly being used for research and regulatory purposes. While the opportunity for increased human relevance of in vitro inhalation methods compared to in vivo tests has been established and discussed, how to

Fabrication of 3D Printed Hydroxyapatite Composite Scaffolds for Bone Regeneration

May 19, 2021
Author(s)
Yoontae Kim, Eun-Jin Lee, Albert Davydov, Stanislav Frakhtbeyen, Jonathan Seppala, Laurence Chow, Tagaki Shozo, Stella Alimperti
Additive biomanufacturing has been adapted in a wide variety of biomedical and tissue engineering applications, including orthopedics. The ability to print biocompatible, patient-specific geometries with controlled porosity, mechanical strength has made

The NIH Somatic Cell Genome Editing program

April 7, 2021
Author(s)
Samantha Maragh
The move from reading to writing the human genome offers new opportunities to improve human health. The United States National Institutes of Health (NIH) Somatic Cell Genome Editing (SCGE) Consortium aims to accelerate the development of safer and more

Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19

March 8, 2021
Author(s)
Linhua Tian, Elzafir B. Elsheikh, Paul Patrone, Anthony Kearsley, adolfas Gaigalas, Sarah L. Inwood, Sheng Lin-Gibson, Dominic Esposito, Lili Wang
Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow

Single-cell measurement of plasmid copy number and promoter activity

March 5, 2021
Author(s)
Bin Shao, Jayan Rammohan, Daniel Anderson, Nina Alperovich, David J. Ross, Christopher Voigt
We have developed a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of

Organizing genome engineering for the gigabase scale

February 9, 2021
Author(s)
Bryan A. Bartley, Jacob Beal, Jonathan R. Karr, Elizabeth Strychalski
Engineering an organism's entire genome enables large-scale changes in organization, function, and environmental interactions, with significant implications for industrial, medical, and environmental ap- plications broadly. Improvements to DNA synthesis

Matching and comparing objects in a serial cytometer

October 4, 2020
Author(s)
Nikita Podobedov, Matthew DiSalvo, Jason A. Hsu, Paul Patrone, Gregory A. Cooksey
Flow cytometers are indispensable for clinical studies, yet are hindered by inherent uncertainties. We have developed an optofluidic device capable of multiple measurements along a microfluidic channel, whereby many of the uncertainty components can be

Serial microfluidic cytometry with inertial and hydrodynamic flow focusing

October 4, 2020
Author(s)
Matthew DiSalvo, Paul Patrone, Gregory A. Cooksey
Microfluidics are increasingly used to develop flow cytometers with novel functionalities. Although various approaches exist to control particle positioning within microfluidics, the magnitude and mechanisms of measurement uncertainties that arise from

Probing Pluripotency Gene Regulatory Networks with Live Cell Imaging

September 20, 2020
Author(s)
Anne Plant, Michael Halter, Jeffrey R. Stinson
Live cell imaging uniquely enables the measurement of dynamic events in single cells, but it has not been used often in the study of gene regulatory networks. Network components can be examined in relation to one another by quantitative live cell imaging

Measurement and Standardization Challenges for Exosome-Based Delivery Vectors

September 3, 2020
Author(s)
Bryant Nelson, Hao-Wei Wang, Samantha Maragh, Paul DeRose, Elzafir B. Elsheikh, Wyatt Vreeland, Ionita Ghiran, Jennifer Jones
Extracellular vesicles (EVs), and in particular exosomes, have the potential to revolutionize the development and efficient delivery of clinical therapeutics. In this Perspective, we focus on providing a brief introduction to the landscape of exosome-based

Tissue Engineering Measurands

August 26, 2020
Author(s)
Greta Babakhanova, Carl Simon, Deepika Arora
A measurand is defined as the quantity that one intends to measure. As tissue engineering research translates into medical products, companies must prepare regulatory filings that contain many different types of measurement data that are collected about

The C-terminus and Third Cytoplasmic Loop Cooperatively Activate Mouse Melanopsin Phototransduction

July 21, 2020
Author(s)
Juan C. Valdez-Lopez, Stephen T. Petr, Matthew P. Donohue, Robin J. Bailey, Meheret Gebreeziabher, Evan G. Cameron, Julia B. Wolf, Veronika Szalai, Phyllis R. Robinson
Melanopsin, an atypical vertebrate visual pigment, mediates non-image forming light responses including circadian photoentrainment and pupillary light reflexes, and contrast detection for image formation. Melanopsin-expressing intrinsically photosensitive

Quantitative Bright-Field Microscopy Combined with Deep Neural Networks Predict Live Tissue Function

February 29, 2020
Author(s)
Carl Simon, Nicholas J. Schaub, Petru S. Manescu, Sarala Padi, Mylene Simon, Peter Bajcsy, Nathan A. Hotaling, Joe Chalfoun, Mohamed Ouladi, Qin Wan, Kapil Bharti, Ruchi Sharma
Progressive increases in the number of cell therapies in the preclinical and clinical phases has prompted the need for reliable and non-invasive assays to validate transplant function in clinical biomanufacturing. Here, we developed a robust
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