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Single-cell measurement of plasmid copy number and promoter activity

Published

Author(s)

Bin Shao, Jayan Rammohan, Daniel Anderson, Nina Alperovich, David J. Ross, Christopher Voigt

Abstract

We have developed a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.
Citation
Nature Communications

Keywords

Synthetic Biology, Biophysics, Design Automation, Metrology, Abstraction, Standards

Citation

Shao, B. , Rammohan, J. , Anderson, D. , Alperovich, N. , Ross, D. and Voigt, C. (2021), Single-cell measurement of plasmid copy number and promoter activity, Nature Communications, [online], https://doi.org/10.1038/s41467-021-21734-y, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=930181 (Accessed March 1, 2024)
Created March 5, 2021, Updated October 12, 2021