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Single-cell measurement of plasmid copy number and promoter activity
Published
Author(s)
Bin Shao, Jayan Rammohan, Daniel Anderson, Nina Alperovich, David J. Ross, Christopher Voigt
Abstract
We have developed a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.
Shao, B.
, Rammohan, J.
, Anderson, D.
, Alperovich, N.
, Ross, D.
and Voigt, C.
(2021),
Single-cell measurement of plasmid copy number and promoter activity, Nature Communications, [online], https://doi.org/10.1038/s41467-021-21734-y, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=930181
(Accessed October 1, 2025)