Serial microfluidic cytometry with inertial and hydrodynamic flow focusing
Matthew DiSalvo, Paul N. Patrone, Gregory A. Cooksey
Microfluidics are increasingly used to develop flow cytometers with novel functionalities. Although various approaches exist to control particle positioning within microfluidics, the magnitude and mechanisms of measurement uncertainties that arise from particle positioning within microfluidic channels remains an open question. Here, we report a microfluidic flow cytometer that combines 3D-hydrodynamic and inertial focusing to confine particles while obtaining velocimetry and quadruple replicate measurements of fluorescence. Compared to inertial focusing alone, this combined focusing approach demonstrated lower particle velocity coefficient of variation (CV) (0.3 %), a lower estimate of particle population CV (9.4 %), and lower replicate measurement CVs (1.4 %).
24th International Conference on Miniaturized Systems for Chemistry and Life Sciences