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DUAL DIELECTROPHORETIC ASSEMBLY OF CO-CULTURES FOR THE STUDY OF CELL MIGRATION INDUCED BY PHYSICAL INTERACTIONS

Published

Author(s)

Brian J. Nablo, Darwin Reyes-Hernandez

Abstract

We present the differences in cell migration patterns of endothelial cells when they physically interact with cancer cells through a porous membrane barrier (1.2 µm pore size). Cells were dielectrophoretically trapped on opposite sides of an 11 µm thick polyester membrane assembled between two microfluidic channels perpendicular to each other. Endothelial cells at the crossover area, where they interact with the HepG2 cells, had larger displacements than cells away from that region. However, when endothelial cells were in monocultures at the crossover area and away from it they show no significant difference in their cell migration behavior.
Proceedings Title
Proceedings of The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021)
Conference Dates
October 10-14, 2021
Conference Location
Palm Springs, CA, US
Conference Title
The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021)

Keywords

Cell migration, Endothelial cells, Cancer cells, Dielectrophoresis, Polyester (PET) membranes

Citation

Nablo, B. and Reyes-Hernandez, D. (2021), DUAL DIELECTROPHORETIC ASSEMBLY OF CO-CULTURES FOR THE STUDY OF CELL MIGRATION INDUCED BY PHYSICAL INTERACTIONS, Proceedings of The 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2021), Palm Springs, CA, US, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=933120 (Accessed August 19, 2022)
Created October 10, 2021, Updated June 16, 2022