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Search Publications by: John Butler (Fed)

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Displaying 76 - 100 of 112

U.S. Population Data for the Multi-Copy Y-STR Locus DYS464

June 25, 2005
Author(s)
John M. Butler, R Schoske
Population studies are important in forensic science and human identity testing to help demonstrate that STR markers are polymorphic and will help distinguish the various individuals from one another. A total of 679 male DNA samples from 3 U.S. populations

Results From the NIST 2004 DNA Quantitation Study

May 1, 2005
Author(s)
Margaret C. Kline, David L. Duewer, Janette W. Redman, John M. Butler
For optimal DNA Short Tandem Repeat (STR) typing results, the DNA concentration ([DNA]) of the sample must be accurately determined prior to the polymerase chain reaction (PCR) amplification step in the typing process. In early 2004, the National Institute

Mitochondrial DNA Typing Screens With Control Region and Coding Region SNPs

March 1, 2005
Author(s)
Margaret C. Kline, Peter M. Vallone, Janette W. Redman, David L. Duewer, C D. Calloway, John M. Butler
Mitochondrial DNA (mtDNA) analysis has found an important niche in forensic DNA typing. It is used with highly degraded samples or low-copy number materials such as might be found from shed hair or bones exposed to severe environmental conditions. The

Multiplexed Assays for Evaluation of Y-SNP Markers in U.S. Populations

April 1, 2004
Author(s)
Peter Vallone, John Butler
Genetic markers located on the Y chromosome are of increasing importance in human identity testing. In an effort to evaluate the forensic utility of Y chromosome single nucleotide polymorphism (SNP) markers, we constructed several novel multiplex allele

Duplication of DYS19 Flanking Regions in Other Parts of the Y Chromosome

March 25, 2004
Author(s)
John M. Butler, R Schoske
During the testing of alternative primers for the Y chromosome short tandem repeat marker DYS19, a duplicated region of the Y chromosome was discovered. The duplicated sequence is contained within GenBank accession AC006335 and has a high degree of

Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

October 17, 2003
Author(s)
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390

A Novel Multiplex for Simultaneous Amplification of 20 Y Chromosome STR Markers

September 10, 2002
Author(s)
John M. Butler, R Schoske, Peter M. Vallone, Margaret C. Kline, A J. Redd, M F. Hammer
A multiplex PCR assay capable of simultaneously amplifying 20 Y-chromosome short tandem repeat (STR) markers has been developed to aid human identity testing and male population studies. These markers include all of the Y STRs that make up the extended

Polymerase Chain Reaction Amplification of DNA From Aged Blood Stains: Quantitative Evaluation of the Suitability for Purpose of Four Filter Papers as Archival Media

April 15, 2002
Author(s)
Margaret C. Kline, David L. Duewer, Janette W. Redman, John M. Butler, D A. Boyer
In collaboration with the Armed Forces Institute of Pathology's Department of Defense DNA Registry, the National Institute of Standards and Technology recently evaluated the performance of a short tandem repeat (STR) multiplex with dried whole blood stains
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