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A Novel Multiplex for Simultaneous Amplification of 20 Y Chromosome STR Markers



John M. Butler, R Schoske, Peter M. Vallone, Margaret C. Kline, A J. Redd, M F. Hammer


A multiplex PCR assay capable of simultaneously amplifying 20 Y-chromosome short tandem repeat (STR) markers has been developed to aid human identity testing and male population studies. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390, DYS391, DYS392, DYS393, and YCAII) plus additional polymorphic Y STRs (DYS437, DYS438, DYS439, DYS447, DYS448, DYS388, DYS426, GATA A7.1, and GATA H4). Primers for the markers DYS385, DYS389, and YCAII target duplicated regions of the Y chromosome and thus can provide two polymorphic peaks for each respective primer set. This Y STR 20plex, which utilizes 34 different PCR primers, is the first to include a simultaneous amplification of all the markers within the European minimal and extended haplotypes. Relative primer positions are compared between the newly developed primers described here and previously published ones. Efforts were made to avoid X chromosome homology in the primer design as well as close packing of PCR product size ranges through careful examination of known allele ranges. Haplotype comparisons between the 20plex and a commercial available kit found excellent agreement across the 76 samples in the Y chromosome consortium panel.
Forensic Science International
No. 1


DNA typing, DYS19, DYS388, DYS389, DYS390, DYS391, DYS392, DYS393, DYS426


Butler, J. , Schoske, R. , Vallone, P. , Kline, M. , Redd, A. and Hammer, M. (2002), A Novel Multiplex for Simultaneous Amplification of 20 Y Chromosome STR Markers, Forensic Science International (Accessed April 23, 2024)
Created September 10, 2002, Updated February 17, 2017