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High-Throughput Y-STR Typing of U.S. Populations With 27 Regions of the Y Chromosome Using Two Multiplex PCR Assays



R Schoske, Peter M. Vallone, Margaret C. Kline, Janette W. Redman, John M. Butler


Two Y-chromosome short tandem repeat (STR) multiplex polymerase chain reaction (PCR) assays were used to generate haplotypes for 19 single copy and 3 multi-copy Y-STRs. A total of 27 PCR products were examined in each sample using the following loci: DYS19, DYS385 a/b, DYS388, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS426, DyS437, DYS438, DYS439, DYS447, DYS448, DYS450, DYS456, DYS458, DYS460, DYS464 a/b/c/d, H4, and YCAII a/b. The first multiplex is the Y-STR 20plex previously described by Butler et al. [Forensic Sci Int. 129 (2002) 10-24]. The second multiplex is a novel Y-STR 11plex and includes DYS385 a/b, DYS447, DYS448 and the new markers DYS450, DYS456, DYS458, and DYS464 a/b/c/d. These two multiplexes were tested on 647 males from 3 United States population sample sets: 260 African Americans, 244 Caucasians, and 143 Hispanics. Haplotype comparisons between common loci included in the 20plex and 11plex assays as well as commercially available kits found excellent agreement across a sampling of the population samples. The multi-copy loci DYS464, DYS385, and YCAII were the most polymorphic followed by the following single copy Y STRs: DYS458, DYS390, DYS447, DYS389II, DYS448, and DYS456. Samples containing the most common type in the European database could be well resolved with additional markers beyond the minimal haplotype loci.
Forensic Science International


DNA typing, multiplex PCR, U.S. populations, Y-chromosome, Y-STR


Schoske, R. , Vallone, P. , Kline, M. , Redman, J. and Butler, J. (2004), High-Throughput Y-STR Typing of U.S. Populations With 27 Regions of the Y Chromosome Using Two Multiplex PCR Assays, Forensic Science International (Accessed May 27, 2024)


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Created January 28, 2004, Updated February 17, 2017