Multiplexed Assays for Evaluation of Y-SNP Markers in U.S. Populations

Published

April 1, 2004

Author(s)

Peter Vallone, John Butler

Abstract

Genetic markers located on the Y chromosome are of increasing importance in human identity testing. In an effort to evaluate the forensic utility of Y chromosome single nucleotide polymorphism (SNP) markers, we constructed several novel multiplex allele-specific primer extension (ASPE) assays and utilized a new commercial allele-specific hybridization (ASH) multiplex kit to examine 50 Y-SNP markers in 229 males from two U.S. Caucasian and African American populations. The novel ASPE assays covered 18 Y-SNP markers in 3 multiplex reactions while a commercial ASH kit was used to type 42 Y-SNPs plus amelogenin for sex-typing purposes. There were 10 overlapping loci between the ASPE and ASH methods permitting an evaluation of concordance on over 2,000 allele calls. The 50 Y-SNP markers examined in this study define 45 of the 159 possible Y Chromosome Consortium (YCC) haplogroups. Only 18 different haplogroups were observed in our samples.

Proceedings Title

International Congress Series; Progress in Forensic Genetics 10

Volume

1261

Conference Dates

September 9-13, 2003

Conference Location

Arcachon, FR

Conference Title

20th International Congress of the International-Society-for-Forensic-Genetics

Pub Type

Conferences

Keywords

allele-specific hybridization, forensic science, primer extension, single nucleotide polymorphism, SNP typing, Y chromosome, Y-SNPs

Citation

Vallone, P. and Butler, J. (2004), Multiplexed Assays for Evaluation of Y-SNP Markers in U.S. Populations, International Congress Series; Progress in Forensic Genetics 10, Arcachon, FR (Accessed October 16, 2025)