NIST Mixed Stain Study #3: DNA Quantitation Accuracy and its Influence on Short Tandem Repeat Multiplex Signal Intensity

Published

May 15, 2003

Author(s)

Margaret C. Kline, David L. Duewer, Janette W. Redman, John M. Butler

Abstract

The Mixed Stain Study #3 (MSS3) interlaboratory challenge exercise evaluated the 2001 performance of STR multiplex DNA typing systems using a set of seven DNA extracts of designed concentration and composition. This initial report focuses on the linkages connecting the measurement of the concentration of DNA ([DNA]) to the observed STR multiplex signal intensities. There is a causal relationship between [DNA] measurement accuracy and the efficiency of STR multiplex analysis. There are no intrinsic measurement performance differences among the [DNA] measurement technologies reported. However, there are large differences in the efficiencies of amplification, separation, and detection among participants using the same nominal measurement systems.

Citation

Analytical Chemistry

Volume

75

Issue

10

Pub Type

Journals

Keywords

DNA fingerprinting, DNA typing, Forensic Science, Interlaboratory comparison, polymerase chain reaction (PCR), short tandem repeat (STR), STR multiplex

Citation

Kline, M. , Duewer, D. , Redman, J. and Butler, J. (2003), NIST Mixed Stain Study #3: DNA Quantitation Accuracy and its Influence on Short Tandem Repeat Multiplex Signal Intensity, Analytical Chemistry (Accessed October 8, 2025)