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NIST Mixed Stain Study #3: DNA Quantitation Accuracy and its Influence on Short Tandem Repeat Multiplex Signal Intensity

Published

Author(s)

Margaret C. Kline, David L. Duewer, Janette W. Redman, John M. Butler

Abstract

The Mixed Stain Study #3 (MSS3) interlaboratory challenge exercise evaluated the 2001 performance of STR multiplex DNA typing systems using a set of seven DNA extracts of designed concentration and composition. This initial report focuses on the linkages connecting the measurement of the concentration of DNA ([DNA]) to the observed STR multiplex signal intensities. There is a causal relationship between [DNA] measurement accuracy and the efficiency of STR multiplex analysis. There are no intrinsic measurement performance differences among the [DNA] measurement technologies reported. However, there are large differences in the efficiencies of amplification, separation, and detection among participants using the same nominal measurement systems.
Citation
Analytical Chemistry
Volume
75
Issue
10

Keywords

DNA fingerprinting, DNA typing, Forensic Science, Interlaboratory comparison, polymerase chain reaction (PCR), short tandem repeat (STR), STR multiplex

Citation

Kline, M. , Duewer, D. , Redman, J. and Butler, J. (2003), NIST Mixed Stain Study #3: DNA Quantitation Accuracy and its Influence on Short Tandem Repeat Multiplex Signal Intensity, Analytical Chemistry (Accessed June 24, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created May 14, 2003, Updated June 24, 2021