Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

Published

October 17, 2003

Author(s)

John M. Butler, R Schoske, Peter Vallone

Abstract

A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390, SYS391, DYS392, DYS393, and YCAII) plus additional polymorphic Y STRs DYS437, DYS438, DYS439, DYS447, DYS448, DYS388, DYSS426, GATA A7.1, and GATA H4. Single nucleotide polymorphism (SNP) assays that may be rapidly analyzed with time-of-flight mass spectrometry have also been developed using Y chromosome bi-allelic markers. With improved multiplexes and techniques for more rapid data collection such as mass spectrometry, Y chromosome haplotypes maybe more quickly generated for DNA databases and population studies and forensic cases may be solved more efficiently.

Proceedings Title

International Congress Series: Progress in Forensic Genetics

Volume

9

Issue

1239

Conference Dates

August 28-September 1, 2001

Conference Location

Munster

Conference Title

19th International Congress of the International-Society-Forensic-Genetics

Pub Type

Conferences

Keywords

DNA typing, Y chromosome testing, Y SNPs, Y STRs

Citation

Butler, J. , Schoske, R. and Vallone, P. (2003), Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome, International Congress Series: Progress in Forensic Genetics, Munster, -1 (Accessed October 14, 2024)