Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390, SYS391, DYS392, DYS393, and YCAII) plus additional polymorphic Y STRs DYS437, DYS438, DYS439, DYS447, DYS448, DYS388, DYSS426, GATA A7.1, and GATA H4. Single nucleotide polymorphism (SNP) assays that may be rapidly analyzed with time-of-flight mass spectrometry have also been developed using Y chromosome bi-allelic markers. With improved multiplexes and techniques for more rapid data collection such as mass spectrometry, Y chromosome haplotypes maybe more quickly generated for DNA databases and population studies and forensic cases may be solved more efficiently.
International Congress Series: Progress in Forensic Genetics
August 28-September 1, 2001
19th International Congress of the International-Society-Forensic-Genetics
, Schoske, R.
and Vallone, P.
Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome, International Congress Series: Progress in Forensic Genetics, Munster, -1
(Accessed December 4, 2023)