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Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome



John M. Butler, R Schoske, Peter Vallone


A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390, SYS391, DYS392, DYS393, and YCAII) plus additional polymorphic Y STRs DYS437, DYS438, DYS439, DYS447, DYS448, DYS388, DYSS426, GATA A7.1, and GATA H4. Single nucleotide polymorphism (SNP) assays that may be rapidly analyzed with time-of-flight mass spectrometry have also been developed using Y chromosome bi-allelic markers. With improved multiplexes and techniques for more rapid data collection such as mass spectrometry, Y chromosome haplotypes maybe more quickly generated for DNA databases and population studies and forensic cases may be solved more efficiently.
Proceedings Title
International Congress Series: Progress in Forensic Genetics
Conference Dates
August 28-September 1, 2001
Conference Location
Conference Title
19th International Congress of the International-Society-Forensic-Genetics


DNA typing, Y chromosome testing, Y SNPs, Y STRs


Butler, J. , Schoske, R. and Vallone, P. (2003), Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome, International Congress Series: Progress in Forensic Genetics, Munster, -1 (Accessed June 22, 2024)


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Created October 16, 2003, Updated February 19, 2017