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Standards

Reference materials

Projects/Programs

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Recertification of Neutral Density Standard Reference Materials for Regular Spectral Transmittance

Ongoing

Certifications and recertifications for SRMs 930x, 1930, 2930, and 2031x expire after two years. To have a filter set recertified, go to https://shop.nist.gov/ or go to nist.gov, click on ‘Shop’ in the upper right corner, then click on ‘Calibration Services’. Follow the instructions on the website...

Reference Material 8038 H5N1 (Avian Influenza) Synthetic RNA Fragments

Completed

In response to the H5N1 avian influenza outbreak, NIST has developed a Reference Material (RM) for PCR-based assays. This material contains 3 vials with RNA fragments for HA (Part A), NA (Part B), and MP (Part C). The Reference Material Information Sheet (RMIS) for this material contains information...

Human Vitamin D Binding Protein in complex with 25-hydroxyvitamin D3

Reference Materials and Methods for Vitamin D Binding Protein

Ongoing

Vitamin D is essential for bone health, and vitamin D deficiency can lead to rickets in children and osteomalacia (soft bones) in adults. Vitamin D status is typically monitored through measurement of the metabolite 25-hydroxyvitamin D [25(OH)D] in serum. Most 25(OH)D is tightly bound to its...

Reference Materials and Standards for Fossil Fuels, Electric Utility Emissions, and Coal Combustion By-Products

Ongoing

Until newer sustainable energy technologies can be deployed, fossil fuels will remain the principal source of energy for the developed world. Coal, oil, and natural gas account for over 85 % of the energy consumed by the U.S. Along with the need for more efficient utilization of these resources...

Relative Intensity Correction Standards for Fluorescence and Raman Spectroscopy

Ongoing

Intended Impact The Raman-based detection and identification of bio-agents and other chemical species depends on the measurement of relative intensities of Raman signal peaks with varying Raman shift. Similarly, the accuracy of fluorescence-based, quantitative clinical and biochemical assays depends...

Renewal of NIST Benchmark SRMs for Accurate Determinations of Trace Elements in Serum and Liver Tissues

Completed

NIST has maintained two benchmark materials, a fresh-frozen animal serum SRM and a freeze-dried powdered bovine liver tissue, SRM 1598 Bovine Serum and SRM 1577b Bovine Liver, respectively. The renewals of these materials had to address the challenges of very low levels of trace elements typically...

RMAT Laboratory Programs

Ongoing

Examples of Current Activities: Cell Counting and viability are key measurements critical for decision making from R&D to manufacturing. We recently developed an approach for evaluating the quality of cell counting measurements through experimental design and statistical analysis that does not...

Saccharomyces cerevisiae (RM 8230): A NIST Reference Material Supporting Research and Quality Assurance in Multiple Microbial Fields

Ongoing

Quantification of total and viable microbial cells is essential across a wide range of scientific and industrial applications—from evaluating DNA extraction efficiency in support of microbial DNA-based techniques to validating antimicrobial treatments and benchmarking technologies for microbial...

Epidemiologist in protective suit, mask and glasses works with patient swabs to detect specific region of 2019-nCoV virus causing Covid-19

SARS-CoV-2 Research Grade Test Material

Completed

In response to the COVID-19 pandemic, the National Institute of Standards and Technology released a synthetic RNA material for SARS-CoV-2 in June 2020. The goal was to rapidly produce a material to support molecular diagnostic testing applications. This material, referred to as Research Grade Test...

Scanning Probe Microscopy Calibrations and Standards

Ongoing

The Nanomechanical Properties Group has expertise in a wide variety of SPM techniques, especially atomic force microscopy (AFM); however, the main research thrust for the Group’s standards project area deals with accurate cantilever stiffness calibration and the use and development of AFM methods...

Sensing and Imaging of concealed objects

Ongoing

Threats to personal and collective safety abound in our society. The Security Technologies Group (STG) performs research to advance the technology and measurement science of security imaging and sensing. This work includes the establishment of test and evaluation tools, reference test beds, test...

Solvation Thermodynamics

Ongoing

Solvation thermodynamics data are essential in the characterization and interpretation of any process carried out in the liquid phase. The data have application in the study of biochemical processes such as protein folding and protein-protein association in aqueous fluids and are useful as...

Standard Solution SRMs Provide Traceability for Millions of Measurements

Ongoing

Literally millions of measurements of elemental composition are performed each year worldwide. The vast majority of these measurements depend upon reference materials for establishing traceability of results to the SI. Leveraged through commercial CRM producers, the NIST SRM 3100 series provides an...

DNA Plasmid containing 13 PCR targets for water quality testing

Standards for Assessing the Safety of Recreational Waters from Fecal Contamination

Completed

Over 286 million Americans get their tap water from a community water system. To assure the water safety, bacterial testing must be performed regularly. Major sources of contaminants include human derived sources, such as sewage leaks or combined sewer overflows; and agricultural sources, such as...

Standards Development to Ensure Reliable Breath Analysis in the Field

Ongoing

Numerous volatile organic compounds (VOCs) have been identified in human breath. These compounds can be produced by the body or by organisms in the body (e.g., bacteria or viruses) and provide a non-invasive window into human health. Inexpensive point-of-care devices are being developed to diagnose...

Standards for Metagenomics

Ongoing

Metagenomics is a powerful technology combining high-throughput (a.k.a. “next-generation”) sequencing with advanced bioinformatics to identify and quantify mixtures of organisms in a sample. Metagenomics workflows consist of 4 main steps: Sample collection: location, amount, storage, etc. Extraction...

Standards to Support Biological Detection in the Field

Ongoing

NIST is working in partnership with the Department of Homeland Security Science and Technology Directorate (DHS S&T) to develop methods, guidance, standards, and reference materials to support and improve our Nation's ability to detect and respond to suspected biothreat incidents. Specific...

First responders collecting samples of the yeast surrogate material during a field exercise. (Credit: NIST)

Standards to Support Biothreat Detection

Ongoing

Since 9/11 and the subsequent anthrax attacks in 2001, suspicious white powder incidents continue to occur within the US, often requiring Federal law enforcement response. Suspect samples are sent to public health laboratories for confirmatory evaluation, which can take hours to weeks to receive...

Storage Stability of Freeze-Dried Proteins

Completed

Intended Impact Work in this project will enable industry to develop rational formulation approaches for freeze-drying, thus facilitating the manufacture safer, longer lasting biopharmaceuticals, with less time and resource cost required for stabilization. Biopharmaceuticals are the fastest growing...

Strain Measurement for Semiconductor Devices

Ongoing

Mechanical strain is hugely important to semiconductor devices and packages while also being difficult to measure accurately. Strain is engineered into CMOS channels to improve carrier mobility for higher performance at lower power but is also intrinsically present from manufacturing processes where...

Study of Microheterogeneity in Solid Reference Materials

Ongoing

Presently there are a variety of analytical techniques that are served by few or no reference materials due to the small sample sizes they require. NIST currently certifies solid reference materials at or above the 100 mg mass level in most cases with no mention of the effect of using smaller sample...

Surrogate Materials to Support Training in Biological Sampling and Detection in the Field

Ongoing

Nationwide, first responders regularly encounter suspicious materials that might be biological threat agents. Routine training and process evaluations help ensure workflows, operators, and technologies are working together properly within the concept of operations. Biological test materials have the...

Surrogate protein particle standards

Ongoing

Intended Impact This work will enable industry to obtain accurate, reproducible counts of protein particles, and to differentiate protein particles from other particle types, resulting in greater drug efficacy and safety. Objectives Create a polydisperse suspension of polymer particles that mimic...

Research Grade Testing Material (RGTM 10212), Synthetic Fecal Metabolite Calibrant (Credit: NIST)

Synthetic Fecal Metabolite Calibrant Solution (RGTM 10212)

Ongoing

Metabolomics is an exciting and dynamic field in life sciences that harnesses cutting-edge technologies to identify and characterize small molecules, or metabolites, within cells, tissues, or organisms, collectively known as the metabolome. The wealth of evidence in the literature underscores the...

US TAG for ISO/TC 334: Reference Materials

Ongoing

Get involved: Interested US Stakeholders may participate in the US TAG at no cost. Membership provides access to all ISO/TC 334 and US TAG documents, including standards under development. Please click here to join or renew your membership. Contribute to standards development : Please click here to...

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