Projects/Programs

Displaying 1 - 25 of 37

RM 8376 Microbial Pathogen DNA Standards for Detection and Identification

Ongoing

RM 8376 consists of 20 individual gDNA components from 19 bacterial strains and 1 human reference genome. Its purpose is to enable developers and regulators to evaluate the analytical performance of metagenomic next generation sequencing (mNGS) tools. The material is certified for chromosomal copy...

AMPHORA (Assured Microbial Preservation in Harsh Or Remote Areas)

Ongoing

The Defense Advanced Research Projects Agency’s Biological Technologies Office (DARPA BTO) has launched the Assured Microbial Preservation in Harsh Or Remote Areas (AMPHORA). The goal of the AMPHORA program is to expand our current capacity to extract and viably maintain pathogens from complex...

Assay Development for Cell-Free Expression Systems

Ongoing

Current practices for characterizing cell-free expression systems are limited and unsuitable to advance these technologies beyond the state-of-the-art. To meet this measurement need, the Cellular Engineering Group is developing quantitative assays to benchmark cell extracts, reagents, and cell-free...

Biofilm Models to Evaluate Structure-Function Relationships

Ongoing

Biofilms are complex 3D microbial structures that exist throughout nature including on our skin and gut mucosa, in waterways, and in soil. They are typically composed of multiple microbial species surrounded by proteins, polysaccharides, and extracellular DNA woven together into a hydrated matrix...

Biosurveillance and Pathogen Detection

Ongoing

NIST has established partnerships with other federal agencies to develop standards and measurement solutions to support biosurveillance. These efforts are supported in part through interagency agreements with partner agencies listed below to address their standards needs. PROGRAMS Standards for...

Combating Antibiotic Resistance

Ongoing

We are developing in vitro systems to generate controlled chemical and community environments that mimic the features of real-world that are important determinants for evolutionary rate and resistance potential. In addition, we are developing tools to isolate and analyze single bacteria along the...

DARPA Friend or Foe (FoF) – Independent Verification and Validation (IV&V)

Completed

Vision Validate new measurement platforms for identifying commensal (friend) and pathogenic (foe) microorganisms Goal Provide well-characterized, microbial community reference materials of increasing complexity Approach Start with well-characterized pure cultures and scale up to complex consortia in...

Development of Enhanced mRNA Amplification Using Microfluidics

Completed

Intended Impact Tissue samples frequently contain multiple cell types. New methods (e.g. laser capture microdissection) enable the segregation of small quantities of individual cells and cell types, but the current methods to analyze global gene expression do not provide highly efficient profiling...

Map of the world with the engineering biology topics covered in Americas, Europe, and Asia meetings

Engineering Biology Metrics and Technical Standards for the Global Bioeconomy

Ongoing

Stakeholders from the Americas, Asia and Australia, and Europe and Africa, came together through a series of regional workshops in Washington D.C., Singapore, and Brussels, respectively, to identify specific areas for development, both technical and non-technical towards continued scale-up and economic growth across the bioeconomy.

Engineering of G Protein-Coupled Receptors

Ongoing

A trait of all life is the ability to sense and respond to changing environments, and the largest family of eukaryotic proteins that sense and respond to extracellular signals are called G Protein-Coupled Receptors (GPCRs). In humans, over 800 GPCRs detect a wide range of biological and chemical...

Human Gut Microbiome Reference Material

Ongoing

The most common measurements currently being used to describe these complex microbial communities are: NGS-based metagenomics Mass spectrometry-based metabolomics In either case, no fit-for-purpose standards exist that enable researchers to compare results generated across different laboratories and...

Large-Scale Genotype-Phenotype Landscape Measurements for Precision Engineering of Living Measurement Systems

Ongoing

Synthetic biology promises an array of world-changing innovations including living therapeutics that fight infections and cancer or even regrow whole organs in situ; cellular factories that weave materials with nanoscale features; and synthetic ecosystems that produce fertilizer and pesticides on...

Measuring Antimicrobial Resistance

Ongoing

NIST scientists are leveraging a wide range of measurement capabilities to study microbial community dynamics with regard to antimicrobial resistance. Using a range of culture, molecular and sequencing methods, we hope to develop methods to better understand antibiotic resistance evolution, transfer...

Absolute quantitation of mRNA molecules in bacterial cells

Methods for Absolute Quantitation of Transcription

Ongoing

The ability to engineer novel, useful functions into microbial cells for manufacturing or therapeutic applications has accelerated rapidly over the last few decades. However, the measurements required to underpin predictive engineering of these systems are typically not comparable across different...

Microbial Cell Quantification and Characterization

Ongoing

Quantifying microbial cells is challenging since microbes vary in size (often < 1 µm in diameter), shape (rod, sphere, etc.) and morphology (single cells, doublets, chains, clusters, etc.), and typically average more than one genome copy per cell. Additionally, microbial samples encompass an...

Microbial Flow Cytometry

Ongoing

Microbial flow cytometry capabilities support numerous ongoing projects, including: Microbial Cell Enumeration and Characterization: Establishing standards, measurement methods, and Reference Materials to enable stakeholders to assess accuracy, bias, and comparability across microbial enumeration...

Microbial Genomic Measurements

Completed

The RM 8375 is a stable and homogeneous material whose intended use is whole genome sequencing quality control and proficiency testing. The genomic DNA is intended to be analyzed in the same way as any other sample a laboratory would analyze extracted DNA, such as through the use of genome assembly...

Figure showing four components of metabolomics with samples and processing

Microbial Metabolomics

Ongoing

Mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy are widely used and complementary analytical platforms for the characterization, identification, and quantification of a metabolome. MS techniques, typically coupled to chromatography, provide a chemical fingerprint of complex...

Mpox (MPXV) Synthetic DNA PCR Standards

Ongoing

A new material from NIST can aid in the evaluation and development of selected qPCR assays for the mpox virus. We are offering a unit free of charge in exchange for your feedback, which will help us improve and further develop the material. Questions? Contact MPXV-RGTM [at] nist.gov (MPXV-RGTM[at]nist[dot]gov) . NIST supports the...

Nanocalorimetry Measurements

Ongoing

Accurate thermodynamic measurements are essential to understand fundamental properties of materials, providing direct and quantifiable insight into the thermodynamics of thin film reactions and phase transitions. Going forward, new classes of materials may only be synthesized as thin films, a scale...

NIST LabCAS: Data Driven Science Architecture and Resource

Ongoing

Biological data lie at the heart of innovation and artificial intelligence (AI) advances in the emerging biotechnology sector and serve as key strategic resources for achieving major breakthroughs in biomanufacturing, as summarized by the National Security Commission on Emerging Biotechnology's...

NIST Living Measurement Systems Foundry

Ongoing

To enable the production of high-throughput, high-quality data that meets the rapidly evolving needs of current and future stakeholders, NIST has established an automation facility for the growth, manipulation, sample preparation, and measurement of engineered microbes. The core of the facility...

NIST Microbial Strain Collection

Ongoing

Microorganisms of interest include but are not limited to those relevant to: biomanufacturing of advanced therapy products; rapid microbial testing; microbial therapeutics (e.g., live biotherapeutic products, probiotics, and fecal microbiota transplantations (FMTs)); infectious disease...

NIST Rapid Microbial Testing Methods Consortium

Ongoing

The safety and quality of advanced therapies, including cell therapy, gene therapy, and tissue engineered products, must be maintained prior to patient administration. The culture-based compendial methods currently used to assess product purity (specifically to ensure absence of microbial...

Pathogen Detection and Identification: The Next Generation of Analyses

Ongoing

Human disease resulting from pathogens represents a tremendous burden on the health care system. Many hospitals have adopted new, rapid polymerase chain reaction (PCR)-based detection systems because the time to diagnose and begin effective treatment has a direct impact on patient survival and...

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