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Summary
A synthetic RNA material based on the H5N1 avian influenza has been developed in collaboration with the US CDC. Currently, this material is available as a Research Grade Test Material, with further information about its properties and how it can be obtained found below.
Description
One unit of RGTM 10263 contains 3 tubes for the H5_HA, N1_NA, and MP RNA fragments.
In response to the current H5N1 avian influenza outbreak, NIST has developed a research grade test material (RGTM) for PCR-based assays. This material contains 3 vials with RNA fragments for HA (Part A), NA (Part B), and MP (Part C). A NIST Reference Material is also under development.
The guidance document for this material contains information about usage and storage, as well as the constituents of the tubes.
Current estimates for the concentration of the three components are given in the table below:
Part | Target RNA | Nominal Concentration (copies/μL) |
|---|---|---|
A | H5_HA | 9.1 x 105 |
B | N1_NA | 8.4 x 105 |
C | MP | 6.6 x 105 |
To Receive RGTM 10263
This material is available through the NIST Office of Reference Materials: https://shop.nist.gov/ccrz__ProductDetails?sku=10263&cclcl=en_US
To Provide Feedback
Please contact h5flu [at] nist.gov (h5flu[at]nist[dot]gov)
To Access Data for RGTM 10263
To download the sequences for the 3 components, download this file.