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Peter M. Vallone (Fed)

Leader, Applied Genetics Group

Peter M. Vallone received his Ph.D. in Chemistry from the University of Illinois at Chicago in 1999.  Afterward, he was awarded an NRC postdoctoral fellowship that brought him to the Biotechnology Division at the National Institute of Standards and Technology (NIST).  Over the last 22 years at NIST, Dr. Vallone has developed multiplex PCR assays for the detection of genetic variation, developed methods for the rapid amplification of STR loci, and has been involved in the characterization of nucleic acid-based reference materials. 

As the leader of the Applied Genetics Group at NIST since 2013, Dr. Vallone works with a team of researchers producing DNA reference materials, assessing emerging techniques such as next-generation sequencing and digital PCR.  The group efforts provide research and training that supports the forensic DNA community.  Dr. Vallone has published over 80 peer-reviewed articles in the areas of DNA thermodynamics and human identity testing.


2021 United States of America Department of Commerce Gold Medal Award for Distinguished Achievement in the Federal Service for the rapid development of a SARS-CoV-2 Research Grade Test Material, RGTM 10169, to assess RNA-based diagnostic tests and benchmark SARS-CoV-2 test control materials.

2020 United States of America Department of Commerce Gold Medal Award for Distinguished Achievement in the Federal Service for developing a suite of human genome metrology tools via a NIST-led consortium to make it possible to decipher life's code with unprecedented rigor.

2020 NIST -  MML Accolade Award (For the rapid development of nucleic acid-based measurement methods and standards, including RGTM 10169: SARS-CoV-2 Synthetic RNA Fragments, to support detection and characterization of the SARS-CoV-2 virus in a cross-division and -OU multidisciplinary, teamwork effort.)

2014  United States of America Department of Commerce Silver Medal Award for Meritorious Achievement in the Federal Service (For the development of rapid forensic DNA typing techniques that enable state-of-the-art human identity testing and DNA biometrics.)

2009 International Society of Forensic Genetics, Best Poster Prize Award

2008  United States of America Department of Commerce Gold Medal Award for Distinguished Achievement in the Federal Service

1999 NRC Post-Doctoral Fellowship National Institute of Standards and Technology


One in seven pathogenic variants can be challenging to detect by NGS: an analysis of 450,000 patients with implications for clinical sensitivity and genetic test implementation

Stephen Lincoln, Tina Hambuch, Justin Zook, Sara Bristow, Kathryn Hatchell, Rebecca Truty, Michael Kennemer, Brian Shirts, Andrew Fellowes, Shimul Chowdhury, Eric Klee, Shazia Mahamdallie, Megan Cleveland, Peter Vallone, Yan Ding, Sheila Seal, Wasanthi DeSilva, Farol Tomson, Catherine Huang Huang, Russell Garlick, Nazneen Rahman, Marc L. Salit, Stephen Kingsmore, Matthew Ferber, Swaroop Aradhya, Robert Nussbaum
Next-generation sequencing (NGS) is widely used and cost-effective. However, depending on the specific methods used, NGS can have limitations with certain

Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2

Peter Vallone, James Huggett, Vladimir Benes, Jeremy Garson, Kathryn Harris, Mikael Kubista, Timothy McHugh, Jacob Moran-Gilad, Tania Nolan, Michael Pfaffl, Marc Salit, Greg Shipley, Jo Vandesompele
Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule
Created May 31, 2018, Updated December 16, 2021