NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Development of a Forensic DNA Research Grade Test Material

Published

Author(s)

Erica Romsos, Lisa Borsuk, Carolyn Steffen, Sarah Riman, Kevin Kiesler, Peter Vallone

Abstract

Advancements in forensic DNA typing technology and methods have resulted in increased sensitivity and, while beneficial, carry the weight of more challenging profile interpretation. In response, the forensic DNA community has often requested more complex reference materials to address commonly encountered measurement and interpretation issues such as complex DNA mixtures, DNA degradation, and PCR inhibition. The National Institute of Standards and Technology (NIST) released Research Grade Test Material 10235: Forensic DNA Typing Resource Samples to support the forensic DNA community. Components include three single source samples, two degraded samples, and three mixture samples. As part of the Research Grade Test Material (RGTM) process, automated methods for bottling, alternative sample tube types, and the addition of carrier RNA for stabilizing low-quantity samples were investigated. This report describes the preparation and stability of this material.
Citation
Journal of Forensic Sciences
Pub Type
Journals

Download Paper

https://doi.org/10.1111/1556-4029.15639
Local Download

Keywords

Reference Materials, Forensic DNA, Validation, Training Samples, STR, PCR, quantification
Forensic genetics

Citation

Romsos, E. , Borsuk, L. , Steffen, C. , Riman, S. , Kiesler, K. and Vallone, P. (2024), Development of a Forensic DNA Research Grade Test Material, Journal of Forensic Sciences, [online], https://doi.org/10.1111/1556-4029.15639, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=958417 (Accessed October 11, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created October 16, 2024, Updated October 18, 2024
Was this page helpful?