Development of a Forensic DNA Research Grade Test Material

Published

October 16, 2024

Author(s)

Erica Romsos, Lisa Borsuk, Carolyn Steffen, Sarah Riman, Kevin Kiesler, Peter Vallone

Abstract

Advancements in forensic DNA typing technology and methods have resulted in increased sensitivity and, while beneficial, carry the weight of more challenging profile interpretation. In response, the forensic DNA community has often requested more complex reference materials to address commonly encountered measurement and interpretation issues such as complex DNA mixtures, DNA degradation, and PCR inhibition. The National Institute of Standards and Technology (NIST) released Research Grade Test Material 10235: Forensic DNA Typing Resource Samples to support the forensic DNA community. Components include three single source samples, two degraded samples, and three mixture samples. As part of the Research Grade Test Material (RGTM) process, automated methods for bottling, alternative sample tube types, and the addition of carrier RNA for stabilizing low-quantity samples were investigated. This report describes the preparation and stability of this material.

Citation

Journal of Forensic Sciences

Pub Type

Journals

Download Paper

https://doi.org/10.1111/1556-4029.15639

Local Download

Keywords

Reference Materials, Forensic DNA, Validation, Training Samples, STR, PCR, quantification

Citation

Romsos, E. , Borsuk, L. , Steffen, C. , Riman, S. , Kiesler, K. and Vallone, P. (2024), Development of a Forensic DNA Research Grade Test Material, Journal of Forensic Sciences, [online], https://doi.org/10.1111/1556-4029.15639, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=958417 (Accessed December 12, 2024)