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Megan Cleveland (Fed)

Research Biologist

Megan received a Ph.D. in Molecular Biology and Human Genetics from the Johns Hopkins School of Medicine, where she studied pancreas development in mice. In her work at NIST, Megan focuses on projects related to clinical diagnostics and biomanufacturing. She is particularly interested in digital PCR and next generation sequencing. 

Megan led the production of NIST SRM 2365 (BK Virus DNA Quantitative Standard) and NIST SRM 2367 (JC Virus DNA Quantitative Standard). She also worked with the Genome in a Bottle (GiaB) consortium to examine results from targeted sequencing panels on GiaB benchmark genomes. Targeted sequencing panels may offer a cost effective way of characterizing a high number of clinically important genomic regions.

During the COVID-19 pandemic, Megan produced Research Grade Test Material (RGTM 10169) for SARS-CoV-2 RNA. She worked with the Public Affairs Office to make a video about the creation of this material. At present, RGTM 10169 has been distributed to over 170 labs in 25 countries.

Improving COVID-19 Testing
Improving COVID-19 Testing
Working alone in the lab, but with remote support from her colleagues, NIST research biologist Megan Cleveland produced synthetic gene fragments from SARS-CoV-2, the virus that causes COVID-19. This material, which is non-infectious and safe to handle, can help manufacturers produce more accurate and reliable diagnostic tests for the disease. Watch this video to learn more about this project and see what it’s like to work in a lab during a pandemic.

Megan is also interested in using NGS for the detection of possible contaminants in biologically produced materials. She co-organized a NIST/FDA workshop in 2019 for Standards for NGS Detection of Viral Adventitious Agents in Biologics and Biomanufacturing. A summary of the output of the workshop was published in 2020. She is the leader of Subgroup B (Standards and Reference Materials) for the Advanced Virus Detection Technologies Interest Group (AVDTIG)

Postdoctoral Research Opportunities

Megan is currently looking for applicants for the following opportunities:

Accurate Nucleic Acid Measurements for Molecular Diagnostics

Ensuring the Safety of Vaccines and Biologics

If you are interesting in applying to either opportunity, please email Dr. Cleveland at megan.cleveland [at] (megan[dot]cleveland[at]nist[dot]gov).

Selected Publications


NIST's Engagement with CCQM Studies from 1992 to 2023: History and Performance

David L. Duewer, Christina Cecelski, Megan Cleveland, Nancy Lin, Katrice Lippa, Jacqueline L. Mann, John L. Molloy, Michael Nelson, James E. Norris, Kenneth W. Pratt, Sumona Sarkar, Hratch G. Semerjian, Robert L. Watters
This report documents the engagement and measurement performance of the National Institute of Standards and Technology (NIST) in studies coordinated by the

Digital PCR for the Characterization of Reference Materials

Megan Cleveland, Hua-Jun He, Mojca Milavec, Young-Kyung Bae, Peter Vallone, Jim Huggett
Well characterized reference materials are essential to ensuring the harmonization and accuracy of nucleic acid-based tests (such as qPCR); digital PCR (dPCR)

Results of German external quality assessment schemes for SARS-CoV-2 antigen detection

Laura Vierbaum, Nathalie Wojtalewicz, Peter Grunert, Anika Zimmerman, Annemarie Scholz, Sabine Goseberg, Patricia Kaiser, Ulf Duehring, Christian Drosten, Victor Max Corman, Daniela Niemeyer, Holger F. Rabenau, Martin Obermeier, Andreas Nitsche, Janine Michel, Jim Huggett, Denise O'Sullivan, Simon Cowen, Peter Vallone, Megan Cleveland, Samreen Falak, Andreas Kummrow, Ingo Schellenberg, Heinz Zeichhardt, Martin Kammel
The COVID-19 pandemic illustrated the important role of diagnostic tests, including lateral flow tests (LFTs), in identifying patients and their contacts to
Created September 17, 2019, Updated December 9, 2022