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Projects/Programs

Keyword

Organizational Unit

Flow cytometers are commercial instruments that provide rapid multiparameter measurements of single cells, which makes them indispensable in basic research and clinical studies of diseases and drug therapies. Measurements of cellular attributes can include indicators of cell size and shape and well

3D Orientation Imaging

Ongoing

We have developed 3D orientation imaging methods for both infrared (IR) and coherent anti-Stokes Raman scattering (CARS) signals. The two approaches are complementary to each other. The IR-based 3D orientation imaging is broadly applicable to any pair of non-parallel IR transition dipole moments

Orthogonal Measurements

Ongoing

Definitions: Orthogonal measurements: Measurements that use different physical principles to measure the same property of the same sample with the goal of minimizing method-specific biases and interferences. Complementary measurements: Measurements that corroborate each other to support the same

Pathogen Detection and Identification: The Next Generation of Analyses

Ongoing

Human disease resulting from pathogens represents a tremendous burden on the health care system. Many hospitals have adopted new, rapid polymerase chain reaction (PCR)-based detection systems because the time to diagnose and begin effective treatment has a direct impact on patient survival and

Peptide Mass Spectral Libraries

Ongoing

Intended Impact Modern mass spectrometers used in the field of proteomics are capable of profiling thousands of peptides in a single experiment. Each of these peptides is fragmented to form a mass spectrum. Therefore, interpretation of these mass spectra is a critical step in the experimental

TOP: Image of fluorescent reference material on microscope. BOTTOM: Charting of microscope control parameters.

Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope

Ongoing

Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other imaging applications, few published methods exist for evaluating and benchmarking the analytical

Performance Metrics for Proteomics

Ongoing

Intended Impact Proteomics experiments designed to identify protein markers specific to a particular disease or disease state rely on repeatable identification of proteins within a control set. Repeatability provides statistical assurance that clear differences between two sets (e.g. sick vs

Photopolymerization for Biomaterials

Completed

This project responds to priorities of National Institute of Dental and Craniofacial Research (NIDCR/NIH) for oral health by establishing clinical guidance/standards for adequate photocuring of restorative dental composites. Also, this project is intended to advance the knowledge relevant to thermal

Point-of-Care Pharmaceutical Manufacturing & Precision Medicine

Ongoing

We are employing quality by design (QbD) and risk assessment (cause-and-effect) approaches to investigate production technologies ( e.g., drop on demand or inkjet printing platforms) and appropriate analytical control strategies for point-of-care manufacture of narrow therapeutic index drugs

Polyelectrolyte Solutions and Gels

Ongoing

Materials Extrusion Additive Manufacturing

Polymer Advanced Manufacturing and Rheology

Ongoing

We are measuring the fundamental processes and material parameters that are critical to understanding and furthering polymers-based AM. These efforts will aid the AM ecosystem through better online monitoring capabilities and developing strategies for materials optimization. In situ measurements of

Protection Against Ionizing Radiation in Extreme Radiation-resistant Microorganisms

Ongoing

Intended impact Ionizing radiation-resistant microorganisms have recently garnered a great deal of attention from scientists seeking to understand the mechanisms underlying the survival abilities of these organisms. Much of the focus has been on mechanisms of cellular repair of radiation-induced DNA

Protein Preservation

Completed

This project is an integral part of a cooperative effort – led by NIST, and partially funded by NIH/ NIBIB – which includes the University of Wisconsin, the University of Colorado, and the University of Connecticut. We use the following approach to develop measurement solutions for biopharmaceutical

Protein rheology measurements

Ongoing

Intended Impact Why does the health-care industry care about rheology and scattering of protein solutions? Operations. Viscosity is a critical and fundamental constitutive input for drug delivery, device design and manufacturing. For example, high viscosity leads to poor syringeability due to high

Protocol for Preparing Fibrillar Collagen Matrices on Untreated Polystyrene Labware (Petri)*: A Potential Reference Extracellular Matrix With Robust and Reproducible Cell Responses

Ongoing

Preparation of matrices on a polystyrene surface 1.) Remove DPBS (1x), DPBS (10x), 0.1 M sodium hydroxide, collagen stock from 4 °C storage. 2.) Setup workspace in sterile hood with an appropriate workflow (example shown in Figure 1). 3.) Prepare Collagen Solution. Note: either mix this quickly, or

Protocols, datasets, and software for engineering biology

Ongoing

Published protocols, datasets, and software for engineering biology from the Cellular Engineering Group. Protocols Romantseva E., Ross D., Alperovich N., Strychalski E., Automated DNA template preparation and quantitation methods, https://www.protocols.io, March 23, 2022, https://dx.doi.org/10.17504

Prototype Cell Assay Measurement Platform (P-CAMP)

Ongoing

A systematic approach to the development of robust biological assay includes identifying and ranking the sources of variability in an assay protocol. With a focus on mammalian cellular assays, the Prototype Cell Assay Measurement Platform ( P-CAMP) laboratory was designed and built to take advantage

Quantitative, Cell-based Immunofluorescence Assays

Completed

Analysis of signaling events within individual cells with microscopy imaging has several advantages over non-imaging techniques. The principal advantage of imaging cytometry over plate reader assays and even other single cell techniques such as flow cytometry is the potential for collection of

Quantitative Flow Cytometry Measurements

Ongoing

Flow cytometry is an essential tool for: basic biotechnological and immunological research, the clinical discovery of potential therapeutics, development and approval of drugs and devices, disease diagnosis, and therapeutic treatment and monitoring For example, flow cytometry is commonly used in pre

Quantitative MRI

Ongoing

Future directions may focus on multimodal imaging, techniques that use MRI as either a base or as a complimentary technique. Multimodal imaging combines information from two or more imaging modalities such as MRI, computed tomography (CT), positron emission tomography (PET), and ultrasound (US)

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Ongoing

The current gold standard for cell viability measurements is the trypan blue assay. Trypan blue dye enters dead cells that have a ruptured membrane. As a result, the dead cells are stained blue. Live cells with an intact membrane, on the other hand, do not absorb the dye molecules and remain

Quantum Bioimaging

Ongoing

Our efforts in BBD are focused on using the quantum nature of light to facilitate enhanced and novel measurement technologies for biological samples. For example, so-called bright squeezed laser sources enable imaging and sensing with less noise than is classically possible. Additionally, entangled

Quantum State-Resolved Spectroscopic Techniques

Ongoing

Terahertz radiation interrogates the lowest frequency vibrational (phonon) modes of biomolecules. These modes characterize the incipient motions for large-scale conformational changes responsible for the backbone flexibility of protein, polynucleotide and polysaccharide. Thus, terahertz spectral

Recertification of Neutral Density Standard Reference Materials for Regular Spectral Transmittance

Ongoing

Certifications and recertifications for SRMs 930x, 1930, 2930, and 2031x expire after two years. To have a filter set recertified, go to https://shop.nist.gov/ or go to nist.gov, click on ‘Shop’ in the upper right corner, then click on ‘Calibration Services’. Follow the instructions on the website

Relative Intensity Correction Standards for Fluorescence and Raman Spectroscopy

Ongoing

Intended Impact The Raman-based detection and identification of bio-agents and other chemical species depends on the measurement of relative intensities of Raman signal peaks with varying Raman shift. Similarly, the accuracy of fluorescence-based, quantitative clinical and biochemical assays depends

Bioscience

Projects/Programs

Optofluidic Cytometry

3D Orientation Imaging

Orthogonal Measurements

Pathogen Detection and Identification: The Next Generation of Analyses

Peptide Mass Spectral Libraries

Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope

Performance Metrics for Proteomics

Photopolymerization for Biomaterials

Point-of-Care Pharmaceutical Manufacturing & Precision Medicine

Polyelectrolyte Solutions and Gels

Polymer Advanced Manufacturing and Rheology

Protection Against Ionizing Radiation in Extreme Radiation-resistant Microorganisms

Protein Preservation

Protein rheology measurements

Protocol for Preparing Fibrillar Collagen Matrices on Untreated Polystyrene Labware (Petri)*: A Potential Reference Extracellular Matrix With Robust and Reproducible Cell Responses

Protocols, datasets, and software for engineering biology

Prototype Cell Assay Measurement Platform (P-CAMP)

Quantitative, Cell-based Immunofluorescence Assays

Quantitative Flow Cytometry Measurements

Quantitative MRI

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Quantum Bioimaging

Quantum State-Resolved Spectroscopic Techniques

Recertification of Neutral Density Standard Reference Materials for Regular Spectral Transmittance

Relative Intensity Correction Standards for Fluorescence and Raman Spectroscopy

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