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Search Publications by: David J. Ross (Fed)

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Displaying 26 - 50 of 62

Multiplexed "Detectorless" Electrophoresis

December 14, 2008
Author(s)
David Ross, Jason Kralj
A new microfluidic electrophoresis device and technique is described that is designed specifically for multiplexed, high throughput separations. The device consists of an array of short (3 mm) capillaries connecting individual sample reservoirs to a common

Electrokinetic Sample Dilution and Concentration in Microfluidics

October 16, 2008
Author(s)
David J. Ross, K G. Olsen, Laurie E. Locascio
A new method is demonstrated which can be used to produce streams of variable, controlled analyte concentration. The method works by electrokinetically manipulating the analyte velocity so that it is different in different parts of a channel. Because of

Contactless Differential Conductivity Detection

October 12, 2008
Author(s)
Gordon A. Shaw, David J. Ross, Steven E. Fick, Wyatt N. Vreeland
We propose a new technique, contactless differential conductivity detection (CDCD,) to improve the detection limit of contactless conductivity detection for capillary and microchannel electrophoresis. By exploiting a 3-electrode differential configuration

Microfluidic DNA Analysis Systems for Forensic Applications

July 18, 2008
Author(s)
Michael Gaitan, Jayna J. Shah, Darwin Reyes-Hernandez, Pierre-Alain Auroux, Jon Geist, Laurie E. Locascio, Wyatt N. Vreeland, David J. Ross, Peter Vallone, Paul Smith, Nicole Morgan, Tom Pohida, John Kakareka, Annelise Barron
This report summarizes the NIST effort on microfluidic DNA analysis systems for forensic applications sponsored by the National Institute of Justice. Currently emerging microfluidics-based forensic systems are implemented in silica (glass) because the

Counter-Flow Gradient Electrofocusing

February 1, 2007
Author(s)
Jonathan G. Shackman, David J. Ross
Counter-flow gradient electrofocusing methods are methods whereby a combination of electrophoresis and a bulk solution counter-flow is used to accumulate or focus analytes at stationary points along a separation column. This review first describes the

Scanning Temperature Gradient Focusing

August 2, 2006
Author(s)
S J. Hoebel, K M. Balss, Barbara J. Jones, C Malliaris, Matt S. Munson, Wyatt N. Vreeland, David J. Ross
Temperature gradient focusing (TGF) is a recently developed technique for the simultaneous concentration and electrophoretic separation of ionic analytes in microfluidic channels. One drawback to TGF as it has previously been described is the limited peak

Micellar Affinity Gradient Focusing

February 25, 2004
Author(s)
K M. Balss, P B. Howell, David J. Ross
This report describes a novel method for the concentration and separation of neutral and/or hydrophobic analytes based on a combination of the analytes partitioning into a micellar phase and their electrophoretic mobility. This method of simultaneous

Chiral Separations by Temperature Gradient Focusing

February 1, 2004
Author(s)
K M. Balss, Wyatt N. Vreeland, Karen W. Phinney, Michael J. Tarlov, David J. Ross
Temperature gradient focusing (TGF) involves application of a temperature gradient across a microchannel or capillary. With an appropriate buffer, the temperature gradient creates a gradient in both the electric field and the electrophoretic velocity

Elastic Flow Instability, Curved Streamlines and Mixing in Microfluidic Flows

February 1, 2004
Author(s)
Jai A. Pathak, David J. Ross, Kalman D. Migler
Flow instabilities are well known to occur in macroscopic flows when elastic fluids flow along curved streamlines. In this work we use flow visualization to study the mechanism underlying a purely elastic flow instability for Poiseuille flow in a micro (m)

Fluorescence Thermometry in Microfluidics

September 29, 2003
Author(s)
David J. Ross, Laurie E. Locascio
Two techniques are described for the measurement of fluid temperatures in microfluidic systems based on temperature-dependent fluorescence. In the first technique, a single, strongly temperature-dependent fluorophore, rhodamine B, is used as the basis for

Effect of Caged Fluorescent Dye on the Electroosmotic Mobility in Microchannels

March 1, 2003
Author(s)
David J. Ross, Laurie E. Locascio
We report on measurements of electroosmotic mobility in polymer microchannels and silica capillaries with and without the addition of a caged fluorescein dye to the buffer. For PMMA microchannels, the mobility was found to increase from (2.6 0.1) 10-4 cm2
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