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Controlled Mixing: An in situ Hybridization Assay of Peptide Nucleic Acids with DNA by Temperature Gradient Focusing

Published

Author(s)

K M. Balss, David J. Ross, H Begley, K G. Olsen, Michael J. Tarlov

Abstract

An in situ nucleic acid hybrdization assay involving DNA and peptide nucleic acids (PNAs) employs temperature gradient focusing to separate and concentrate DNA from PNA/DNA hybrids. The assay is demonstrated in a capillary format but easily incorporated into a lab-on-a-chip format. The fluorescent labeled PNA probe was mixed in situ with a complementary and noncomplementary single-stranded DNA target. The hybridization assay performed by TGF represents a qualitatively new method for mixing in microfluidic formats.
Citation
Journal of the American Chemical Society

Keywords

concentration method, mixing, peptide nucleic acids (PNA), separations

Citation

Balss, K. , Ross, D. , Begley, H. , Olsen, K. and Tarlov, M. (2008), Controlled Mixing: An in situ Hybridization Assay of Peptide Nucleic Acids with DNA by Temperature Gradient Focusing, Journal of the American Chemical Society (Accessed March 4, 2024)
Created October 16, 2008