Controlled Mixing: An in situ Hybridization Assay of Peptide Nucleic Acids with DNA by Temperature Gradient Focusing
K M. Balss, David J. Ross, H Begley, K G. Olsen, Michael J. Tarlov
An in situ nucleic acid hybrdization assay involving DNA and peptide nucleic acids (PNAs) employs temperature gradient focusing to separate and concentrate DNA from PNA/DNA hybrids. The assay is demonstrated in a capillary format but easily incorporated into a lab-on-a-chip format. The fluorescent labeled PNA probe was mixed in situ with a complementary and noncomplementary single-stranded DNA target. The hybridization assay performed by TGF represents a qualitatively new method for mixing in microfluidic formats.
, Ross, D.
, Begley, H.
, Olsen, K.
and Tarlov, M.
Controlled Mixing: An in situ Hybridization Assay of Peptide Nucleic Acids with DNA by Temperature Gradient Focusing, Journal of the American Chemical Society
(Accessed March 4, 2024)