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Scanning Temperature Gradient Focusing



S J. Hoebel, K M. Balss, Barbara J. Jones, C Malliaris, Matt S. Munson, Wyatt N. Vreeland, David J. Ross


Temperature gradient focusing (TGF) is a recently developed technique for the simultaneous concentration and electrophoretic separation of ionic analytes in microfluidic channels. One drawback to TGF as it has previously been described is the limited peak capacity; only a small number of analyte peaks (? 2-3) can be simultaneously focused and separated. In this paper we report on a variation of the TGF method whereby the bulk flow rate is varied over time so that a large number of analytes can be sequentially focused, moved past a fixed detection point, and flushed to waste. In addition to improved peak capacity, the detection limits of the scanning TGF method can be adjusted on-the-fly, as needed for different samples. Finally, scanning TGF provides a technique by which high-resolution, high-peak-capacity electrophoretic separations can be performed in simple, straight, and short microfluidic channels.
Analytical Chemistry


biosystems and health, electrophoresis, microfluidics, temperature gradient focusing


Hoebel, S. , Balss, K. , Jones, B. , Malliaris, C. , Munson, M. , Vreeland, W. and Ross, D. (2006), Scanning Temperature Gradient Focusing, Analytical Chemistry, [online], (Accessed April 16, 2024)
Created August 1, 2006, Updated October 12, 2021