Quantitative Temperature Gradient Focusing Performed Using Background Electrolytes at Various pH Values
Jonathan G. Shackman, Matt S. Munson, C W. Kan, David J. Ross
Temperature gradient focusing (TGF) has previously been shown to be a practical technique for simultaneous concentration and separation of a variety of samples. In this paper we demonstrate that TGF can be conducted at a wide range of pH values. Techniques for first order prediction of the suitability of a given background electrolyte (BGE) for focusing are discussed. Buffer suitability for TGF is assessed experimentally by simultaneously concentrating and separating a pair of fluorescent analytes. One analyte is held at constant concentration for use as an internal standard while the concentration of the other is varied. Peak area is shown to vary linearly with the input dye concentration. A high degree of resolution of similar species is also observed. Temperature gradient focusing and separation was successfully conducted quantitatively in BGE solutions of pH from 3.0 to 10.5.
, Munson, M.
, Kan, C.
and Ross, D.
Quantitative Temperature Gradient Focusing Performed Using Background Electrolytes at Various pH Values, Electrophoresis
(Accessed June 4, 2023)