Projects/Programs

Displaying 1 - 25 of 52

Biofabrication of Tissue Engineered Constructs

Ongoing

OCT imaging was assessed using a model scaffold-cell system consisting of a polysaccharide-based hydrogel seeded with human Jurkat cells. Four test systems were used: hydrogel seeded with live cells, hydrogel seeded with heat-shocked or fixed dead cells and hydrogel without any cells. Time series

Biosurveillance and Pathogen Detection

Ongoing

NIST has established partnerships with other federal agencies to develop standards and measurement solutions to support biosurveillance. These efforts are supported in part through interagency agreements with partner agencies listed below to address their standards needs. PROGRAMS Standards for

Cancer Genome in a Bottle

Ongoing

Goals: This project is an extension of the Genome in a Bottle Consortium to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of cancer genome sequencing to clinical practice and innovations in technologies. The priority of GIAB

Cell Counting for Cell Therapies

Ongoing

Recent Publications and Resources for Cell Counting and Related Measurements Tools NOW AVAILABLE : COMET (Counting Method Evaluation Tool) , A Shiny Application that executes the statistical analysis and reporting outlined in ISO 20391-2:2019 https://cell-counting.shinyapps.io/COMET/ Standards ISO

Cell Viability for Advanced Therapies

Ongoing

In the Biosystems and Biomaterials Division at NIST, we have established several technical programs to support the development and implementation of cell viability assays in advanced therapies. These programs address key needs in cell viability assay development including selection and development

Clinical and Forensic Reference Materials

Ongoing

Below is a listing of the reference materials currently produced by the Applied Genetics group. Clinical Diagnostics SRM 2393 - CAG Repeat Length Mutation in Huntington`s Disease SRM 2365 - BK Virus DNA Quantitative Standard SRM 2366a - Cytomegalovirus DNA (Towne Δ147 BAC) for DNA SRM 2367 - JC

Comparative Mammalian Proteome Aggregator Resource (CoMPARe) Program

Ongoing

Comparative proteomics strives to gain insight into key underlying molecular changes that result in unique phenotypes across related taxa. Proteomic analysis complements comparative genomics by providing evidence of protein abundance, orthogonal to gene copy number and amount of transcript. To

Comparative Serum Proteomics Project

Ongoing

State-of-the-art biomolecular analysis is no longer limited to model organisms and is becoming routine in non-model organisms. Major drivers of this emerging bioanalytical capacity include increasing accessibility and quality of sequenced genomes as well as high-resolution fast-duty cycle mass

DARPA Friend or Foe (FoF) – Independent Verification and Validation (IV&V)

Completed

Vision Validate new measurement platforms for identifying commensal (friend) and pathogenic (foe) microorganisms Goal Provide well-characterized, microbial community reference materials of increasing complexity Approach Start with well-characterized pure cultures and scale up to complex consortia in

Development of the NIST Prototype Advanced Biometrology Laboratory

Ongoing

Currently, NIST is investing in a new type of metrology laboratory that is focused on the unique reference material and high quality assay protocols that are needed in the regenerative medicine and biotechnology communities. The laboratory design is centered on automation that can set up 1000’s of

NIST researcher pipetting solutions into 96-well plate for analysis

Development of Reference Methods and Reference Materials for Protein Biomarkers

Ongoing

Accuracy and reliability of clinical assays is essential for timely and accurate medical interventions. However, studies have shown that large variations in results can occur among routine clinical assays. In one example, a clinical comparison among 13 commercial cardiac troponin I assay platforms

Map of the world with the engineering biology topics covered in Americas, Europe, and Asia meetings

Engineering Biology Metrics and Technical Standards for the Global Bioeconomy

Ongoing

Stakeholders from the Americas, Asia and Australia, and Europe and Africa, came together through a series of regional workshops in Washington D.C., Singapore, and Brussels, respectively, to identify specific areas for development, both technical and non-technical towards continued scale-up and economic growth across the bioeconomy.

Engineering of G Protein-Coupled Receptors

Ongoing

A trait of all life is the ability to sense and respond to changing environments, and the largest family of eukaryotic proteins that sense and respond to extracellular signals are called G Protein-Coupled Receptors (GPCRs). In humans, over 800 GPCRs detect a wide range of biological and chemical

FAQs - Genome in a Bottle

Ongoing

Flow Cytometry Lab

Ongoing

Current Capabilities ERF value assignment measurement service for traceable ERF value assignment to microparticles, Aseptic sorting of cell lines, and primary cells Quantitative measurements of cell surface and intracellular biomarkers by estimating antibody bound per cell Accurate enumeration of

Fluid Suspensions and Emulsions

Ongoing

We aim to: Develop measurement capabilities to relate solution structure and viscosity through novel combinations of unique small volume, high shear rate rheometers with scattering and particle-imaging techniques. A major application of these capabilities is understanding self-association in fluids

viral vector illustration with RNA seen within the capsid

Gene Delivery Particle Analysis with High-Throughput Light Scattering Microscopy

Ongoing

This project aims to develop light-scattering microscopy methods for rapid, multi-attribute characterization of gene therapy particles at the single particle level. Emerging gene therapy treatments use gene delivery particles, such as viral vectors and non-viral carriers, to cure intractable

H5 Influenza Positive Controls

Ongoing

In response to the current H5N1 avian influenza outbreak, NIST has developed a research grade test material (RGTM) for PCR-based assays. This material contains 3 vials with RNA fragments for HA (Part A), NA (Part B), and MP (Part C). A NIST Reference Material is also under development. The guidance

Interdomain Distance Measurements of Monoclonal Antibodies

Ongoing

Through collaboration with researchers at the Institute for Biotechnology and Bioscience Research (IBBR), we have created a library of site-specific spin-labeled and stable isotope-labeled binding partner proteins for the NIST monoclonal antibody (NISTmAb) reference material ( RM8671). Using pulsed

Living Yeast Cell Reference Material (RM 8230)

Ongoing

Quantification of total and viable microbial cells is critical for applications ranging from assessment of DNA extraction efficiency in support of microbial metagenomics to characterization of antimicrobial efficacy. In addition, safe biological materials are needed to substitute for true biothreat

Measurements for minimal cells

Ongoing

Minimal cells are cells whose genome has been stripped of all but those genes essential for replication, in a process called "genome minimization." NIST and collaborators demonstrated genome minimization coupled to single cell imaging as a powerful approach to investigate the minimal genetic

Methods to determine the evolutionary stability of engineered biological function

Ongoing

Advances in engineering biology have greatly expanded our ability to add new and useful functions to living cells. Engineering stable functions has remained a significant challenge, with many different approaches developed to increase the stability, or decrease the variability, of a function over

Metrologies for Protein Structure, Dynamics and Function

Ongoing

Based on its established expertise, infrastructure, and resources for structure/function studies of biological macromolecules, the program aims to impact advances in physics, chemistry, and engineering relevant to biological problems. NIST researchers also develop new technologies for application to

Gears illustrating NIST's cell-free systems initiatives

Metrology For Cell-Free Expression Systems

Ongoing

Cell-free expression systems are poised to advance the US bioeconomy and play a key role in ensuring US manufacturing resilience. NIST has several, on-going projects in its growing portfolio to promote reproducibility, new measurement methods, and best practices and standards in cell-free expression

Figure showing four components of metabolomics with samples and processing

Microbial Metabolomics

Ongoing

Mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy are widely used and complementary analytical platforms for the characterization, identification, and quantification of a metabolome. MS techniques, typically coupled to chromatography, provide a chemical fingerprint of complex

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