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Bioscience

Biometrology

Projects/Programs

Keyword

Organizational Unit

Molecular Innovation in Agriculture

Ongoing

Plant science and breeding aim to develop new plant phenotypes with desired qualities, increased production, disease resistance, and traits that reduce water usage. At the federal level, plant research is conducted by the USDA's Animal and Plant Health Inspection Service (APHIS) and Agricultural

NIST/FDA CVM Evaluation of Assays and Control Materials for Characterizing Genome-Edited Animal Biotechnology Products

Ongoing

FDA CVM oversees regulation of IGAs in animals, including those that have been created using genome editing technologies. Characterization of these IGAs is an important part of the regulatory process to ensure that the edit to the animal’s genome is what it is intended to be and to identify any

NIST Flow Cytometry Standards Consortium 

Ongoing

Advances in cell and gene-based therapeutics as well as other regenerative medicine products have increased the need for high quality, robust, and validated measurements for cell characterization. Flow cytometry, including imaging cytometry, has emerged as an important platform due to its ability to

NIST Genome Editing Consortium: Working Group 1 - Specificity Measurements

Ongoing

Genome editing technologies are able to produce DNA sequence changes of a variety of sizes and at a variety of frequencies in a population of edited cells. Having increased confidence in reliably detecting the sequences generated by a genome editing process was a need area identified. Project 1

NIST Genome Editing Consortium: Working Group 2 - Data & Metadata

Ongoing

Develop a metadata schema for genome editing experiments Develop a way of curating and entry that is low burden for user Release control data with linked metadata PROGRESS Phase1 metadata entries and templates completed In process of compiling and testing use cases, user interfaces, and

NIST Genome Editing Consortium

Ongoing

Targeted genome editing , a method used to alter the DNA of living cells at desired locations, is poised to revolutionize science and medicine. To fight diseases, novel genome edited therapeutics, including those for use in regenerative medicine and infectious diseases, are being developed. Many

NIST Genome Editing Program

Ongoing

For genome editing systems to reach their full potential in research and commercial products, new measurement tools, capabilities, and standards must be developed to efficiently implement and assess the performance of these editing technologies, as well as to evaluate the utility of resulting

NIST Living Measurement Systems Foundry

Ongoing

To enable the production of high-throughput, high-quality data that meets the rapidly evolving needs of current and future stakeholders, NIST has established an automation facility for the growth, manipulation, sample preparation, and measurement of engineered microbes. The core of the facility

Test tube illustrating NIST cell-free workshop topics

NIST Workshops on Cell-Free Expression Systems

Completed

NIST has co-hosted two cell-free workshops. NIST CELL-FREE (Comparable Engineered Living Lysates For Research Education and Entrepreneurship) Workshop (2019) The NIST CELL-FREE workshop took place in La Jolla, CA in February 2019, in collaboration with the J. Craig Venter Institute (JCVI). The

NISTCHO

Ongoing

NISTCHO, a new mammalian cell line of interest to the biopharmaceutical and biomanufacturing communities, is now available from the National Institute of Standards and Technology. This Chinese hamster ovary (CHO) cell line has been engineered by MilliporeSigma to express a non-originator version of

Protein rheology measurements

Ongoing

Intended Impact Why does the health-care industry care about rheology and scattering of protein solutions? Operations. Viscosity is a critical and fundamental constitutive input for drug delivery, device design and manufacturing. For example, high viscosity leads to poor syringeability due to high

Prototype Cell Assay Measurement Platform (P-CAMP)

Ongoing

A systematic approach to the development of robust biological assay includes identifying and ranking the sources of variability in an assay protocol. With a focus on mammalian cellular assays, the Prototype Cell Assay Measurement Platform ( P-CAMP) laboratory was designed and built to take advantage

Quantitative, Cell-based Immunofluorescence Assays

Completed

Analysis of signaling events within individual cells with microscopy imaging has several advantages over non-imaging techniques. The principal advantage of imaging cytometry over plate reader assays and even other single cell techniques such as flow cytometry is the potential for collection of

Quantitative Flow Cytometry Measurements

Ongoing

Flow cytometry is an essential tool for: basic biotechnological and immunological research, the clinical discovery of potential therapeutics, development and approval of drugs and devices, disease diagnosis, and therapeutic treatment and monitoring For example, flow cytometry is commonly used in pre

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Ongoing

The current gold standard for cell viability measurements is the trypan blue assay. Trypan blue dye enters dead cells that have a ruptured membrane. As a result, the dead cells are stained blue. Live cells with an intact membrane, on the other hand, do not absorb the dye molecules and remain

RMAT Laboratory Programs

Ongoing

Examples of Current Activities: Cell Counting and viability are key measurements critical for decision making from R&D to manufacturing. We recently developed an approach for evaluating the quality of cell counting measurements through experimental design and statistical analysis that does not

RNA Computation and Metrology for Engineering Biology

Ongoing

RNA computation RNA computation, i.e., programmed interactions between RNA molecules that process information, is an increasingly useful synthetic biology tool with applications spanning in vitro biosensing, synthetic cells, and cellular control. We have developed cotranscriptionally encoded RNA

Serology and Neutralization Assays for COVID-19

Ongoing

Multiplexed bead-based SARS-CoV-2 serological assay We have developed a validated multiplexed bead-based SARS-CoV-2 serological assay that measures different antibodies produced by a patient (IgG, IgM, IgA). In collaboration with the NIST Applied and Computational Mathematics Division, we have

Single-Cell Manipulation and Measurement

Ongoing

Mammalian cells are an important source material for applications in regenerative medicine, gene therapy, engineering biology, and genome editing. Traditional tools for manipulating mammalian cells operate at a bulk cell population level without precision manipulation of individual cells

Single-molecule biosensors

Ongoing

We are developing new tools to investigate the energy landscape of single-molecule sensors. Our goal is to apply a wide-ranging array of technologies to probe the critical physicochemical properties of nanopore biosensors. These properties include the free energy of confinement for polymers as they

Standards to Support Biological Detection in the Field

Ongoing

NIST is working in partnership with the Department of Homeland Security Science and Technology Directorate (DHS S&T) to develop methods, guidance, standards, and reference materials to support and improve our Nation's ability to detect and respond to suspected biothreat incidents. Specific

First responders collecting samples of the yeast surrogate material during a field exercise. (Credit: NIST)

Standards to Support Biothreat Detection

Ongoing

Since 9/11 and the subsequent anthrax attacks in 2001, suspicious white powder incidents continue to occur within the US, often requiring Federal law enforcement response. Suspect samples are sent to public health laboratories for confirmatory evaluation, which can take hours to weeks to receive

Surrogate Materials to Support Training in Biological Sampling and Detection in the Field

Ongoing

Nationwide, first responders regularly encounter suspicious materials that might be biological threat agents. Routine training and process evaluations help ensure workflows, operators, and technologies are working together properly within the concept of operations. Biological test materials have the

Synthetic Biology

Ongoing

New measurement methods and tools are required for biology to emerge fully as an enabling, practical platform for engineering. The Cellular Engineering Group works to provide a foundation of measurement assurance to support the control and rational design of biological function. Through state of the

Research Grade Testing Material (RGTM 10212), Synthetic Fecal Metabolite Calibrant (Credit: NIST)

Synthetic Fecal Metabolite Calibrant Solution (RGTM 10212)

Ongoing

Metabolomics is an exciting and dynamic field in life sciences that harnesses cutting-edge technologies to identify and characterize small molecules, or metabolites, within cells, tissues, or organisms, collectively known as the metabolome. The wealth of evidence in the literature underscores the

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