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Summary
A synthetic RNA material based on H5N1 avian influenza virus has been developed in collaboration with the US CDC. Currently, this material is available as a NIST Reference Material, with further information about its properties and how it can be obtained found below.
Description
One unit of RM 8038 contains 3 tubes for the H5_HA, N1_NA, and MP RNA fragments.
In response to the H5N1 avian influenza outbreak, NIST has developed a Reference Material (RM) for PCR-based assays. This material contains 3 vials with RNA fragments for HA (Part A), NA (Part B), and MP (Part C).
The Reference Material Information Sheet (RMIS) for this material contains information about usage and storage, as well as the constituents of the tubes.
The concentrations of the three components are given in the table below:
Part | Target RNA | Concentration (copies/μL) |
|---|---|---|
A | H5_HA | 1.738 x 106 |
B | N1_NA | 2.615 x 106 |
C | MP | 1.182 x 106 |
To Order RM 8038
This material is available through the NIST Office of Reference Materials: https://shop.nist.gov/ccrz__ProductDetails?sku=8038&cclcl=en_US
To Provide Feedback
Please contact h5flu [at] nist.gov (h5flu[at]nist[dot]gov)
To Access Data for RM 8038
To download the sequences for the 3 components, download this file.