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Bioscience

Projects/Programs

Keyword

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NIST/NIBIB Medical Imaging Phantom Lending Library

Ongoing

Phantoms are calibration objects that can be used to assess the accuracy, stability, and comparability of medical imaging systems. The NIST/ISMRM MRI system phantom can be used to assess scanner geometric distortion, image uniformity, signal to noise ratio (SNR), resolution, slice profile and...

NIST Rapid Microbial Testing Methods Consortium

Ongoing

The safety and quality of advanced therapies, including cell therapy, gene therapy, and tissue engineered products, must be maintained prior to patient administration. The culture-based compendial methods currently used to assess product purity (specifically to ensure absence of microbial...

NIST Urine Albumin Standardization Program

Ongoing

Kidney Disease – A Global Concern Kidney disease is a significant global public health and economic issue, with Chronic Kidney Disease (CKD) ranking among the top 10 worldwide causes of death. The critical need for early and accurate diagnosis, effective treatment management, and ongoing patient...

Test tube illustrating NIST cell-free workshop topics

NIST Workshops on Cell-Free Expression Systems

Completed

NIST has co-hosted two cell-free workshops. NIST CELL-FREE (Comparable Engineered Living Lysates For Research Education and Entrepreneurship) Workshop (2019) The NIST CELL-FREE workshop took place in La Jolla, CA in February 2019, in collaboration with the J. Craig Venter Institute (JCVI). The...

NIST23: Updates to the NIST Tandem and Electron Ionization Spectral Libraries

Ongoing

NIST Tandem Mass Spectral Library, 2023 release 51,501compounds 60% increase over the 2020 release 399,267 precursor Ions 2.4 million spectra Fragmentation methods 49,590 HRAM (high res accurate mass) compounds 51,292 QTOF, HCD, IT-HRAM, QqQ compounds 49,561 ion trap compounds (low res., up to MS 4)...

NISTCHO

Ongoing

NISTCHO, a new mammalian cell line of interest to the biopharmaceutical and biomanufacturing communities, is now available from the National Institute of Standards and Technology. This Chinese hamster ovary (CHO) cell line has been engineered by MilliporeSigma to express a non-originator version of...

Ontologies and Interoperability in Evolutionary Comparative Analysis

Completed

Intended impact Nearly all scientists who regularly use online resources on genes, proteins and genomes make use of comparative data to advance biomedical research. For instance, researchers often make useful inferences by comparing human genes (as well as proteins, reactions, interactions, pathways...

Optical/electrical particle measurement system

Completed

Intended Impact The results of this work should provide instrument manufacturers with data, methods, and algorithms for enhancing the clarity and accuracy of information their instruments provide to their customers. Users of these instruments (pharma companies) and regulators, will have better...

Optofluidic Cytometry

Ongoing

Flow cytometers are commercial instruments that provide rapid multiparameter measurements of single cells, which makes them indispensable in basic research and clinical studies of diseases and drug therapies. Measurements of cellular attributes can include indicators of cell size and shape and well...

Orthogonal Measurements

Ongoing

Definitions: Orthogonal measurements: Measurements that use different physical principles to measure the same property of the same sample with the goal of minimizing method-specific biases and interferences. Complementary measurements: Measurements that corroborate each other to support the same...

Pathogen Detection and Identification: The Next Generation of Analyses

Ongoing

Human disease resulting from pathogens represents a tremendous burden on the health care system. Many hospitals have adopted new, rapid polymerase chain reaction (PCR)-based detection systems because the time to diagnose and begin effective treatment has a direct impact on patient survival and...

Peptide Mass Spectral Libraries

Ongoing

Intended Impact Modern mass spectrometers used in the field of proteomics are capable of profiling thousands of peptides in a single experiment. Each of these peptides is fragmented to form a mass spectrum. Therefore, interpretation of these mass spectra is a critical step in the experimental...

TOP: Image of fluorescent reference material on microscope. BOTTOM: Charting of microscope control parameters.

Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope

Ongoing

Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other imaging applications, few published methods exist for evaluating and benchmarking the analytical...

Performance Metrics for Proteomics

Ongoing

Intended Impact Proteomics experiments designed to identify protein markers specific to a particular disease or disease state rely on repeatable identification of proteins within a control set. Repeatability provides statistical assurance that clear differences between two sets (e.g. sick vs...

Photopolymerization for Biomaterials

Completed

This project responds to priorities of National Institute of Dental and Craniofacial Research (NIDCR/NIH) for oral health by establishing clinical guidance/standards for adequate photocuring of restorative dental composites. Also, this project is intended to advance the knowledge relevant to thermal...

Point-of-Care Pharmaceutical Manufacturing & Precision Medicine

Ongoing

We are employing quality by design (QbD) and risk assessment (cause-and-effect) approaches to investigate production technologies ( e.g., drop on demand or inkjet printing platforms) and appropriate analytical control strategies for point-of-care manufacture of narrow therapeutic index drugs...

Polyelectrolyte Solutions and Gels

Ongoing

Polymer Advanced Manufacturing and Rheology

Ongoing

The manufacture of polymeric materials, whether from virgin or reclaimed sources, occurs under highly non-equilibrium conditions where temperature and stress fields evolve rapidly. The quality of the materials made from these processes depends not only on the composition of the starting material...

Predicting stability of nucleic acid biopharmaceutical products using advanced computational modeling techniques

Ongoing

Modified oligonucleotides increase efficacy of nucleic acid drugs. These modifications impact degradation resistance, enhance thermodynamic stability, and render oligonucleotides unrecognizable to the immune system. However, not much is known about how these chemical modifications affect structure...

Premise Plumbing Systems for High-Performance Buildings

Ongoing

Objective To develop new measurement methods, modeling approaches, and data to support improvements in building plumbing design, utility consumption, and water quality. Technical Idea The technical idea is to advance understanding of the impacts water use and water-related energy use on its delivery...

Protein Preservation

Completed

This project is an integral part of a cooperative effort – led by NIST, and partially funded by NIH/ NIBIB – which includes the University of Wisconsin, the University of Colorado, and the University of Connecticut. We use the following approach to develop measurement solutions for biopharmaceutical...

Protein rheology measurements

Ongoing

Intended Impact Why does the health-care industry care about rheology and scattering of protein solutions? Operations. Viscosity is a critical and fundamental constitutive input for drug delivery, device design and manufacturing. For example, high viscosity leads to poor syringeability due to high...

Protocol for Preparing Fibrillar Collagen Matrices on Untreated Polystyrene Labware (Petri)*: A Potential Reference Extracellular Matrix With Robust and Reproducible Cell Responses

Ongoing

Preparation of matrices on a polystyrene surface 1.) Remove DPBS (1x), DPBS (10x), 0.1 M sodium hydroxide, collagen stock from 4 °C storage. 2.) Setup workspace in sterile hood with an appropriate workflow (example shown in Figure 1). 3.) Prepare Collagen Solution. Note: either mix this quickly, or...

Protocols, datasets, and software for engineering biology

Ongoing

Published protocols, datasets, and software for engineering biology from the Cellular Engineering Group. Protocols Romantseva E., Ross D., Alperovich N., Strychalski E., Automated DNA template preparation and quantitation methods, https://www.protocols.io, March 23, 2022, https://dx.doi.org/10.17504...

Prototype Cell Assay Measurement Platform (P-CAMP)

Ongoing

A systematic approach to the development of robust biological assay includes identifying and ranking the sources of variability in an assay protocol. With a focus on mammalian cellular assays, the Prototype Cell Assay Measurement Platform ( P-CAMP) laboratory was designed and built to take advantage...

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