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Health

Precision medicine

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Displaying 76 - 100 of 106

Live quantification of cell viability via neutral red uptake using lens-free imaging

October 27, 2019
Author(s)
Brian J. Nablo, Jungjoon Ahn, Kiran Bhadriraju, Jong M. Lee, Darwin Reyes-Hernandez
We present the quantification of cell viability during neutral red (NR) uptake with a compact lens-free system utilizing two light sources. Conventionally, the NR uptake assay determines cell viability based on the accumulation of NR within lysosomes and

Primary standardization of Ra-224 activity by liquid scintillation counting

October 11, 2019
Author(s)
Denis E. Bergeron, Jeffrey T. Cessna, Ryan P. Fitzgerald, Brian E. Zimmerman, Lizbeth Laureano-Perez, Ronald Colle, Leticia S. Pibida, Elisa Napoli
A standard for 224Ra activity has been developed, based on triple-to-double coincidence ratio (TDCR) liquid scintillation (LS) counting. The standard was confirmed by efficiency tracing and 4παβ(LS)-γ(NaI(Tl)) anticoincidence counting, as well as by 4πγ

QUANTUM-LIMITED 2D SENSORS FOR PH AND BIOSENSING

September 27, 2019
Author(s)
Arvind K. Balijepalli, Son T. Le, Harish C. Pant, Curt A. Richter
We have developed biosensors based on dual-gated field-effect transistors (FETs) that operate at the quantum capacitance limit. The FETs are fabricated with atomically thin MoS2 semiconducting films and top-gated with a room temperature ionic-liquid. The

Practical Guidelines to 13C-based NMR Metabolomics

August 29, 2019
Author(s)
Arthur S. Edison, Adrien Le Guennec, Frank Delaglio, Eriks Kupce
We present an overview of 13C-based NMR metabolomics. At first glance, the low sensitivity of 13C relative to 1H NMR might seem like too great an obstacle to use this approach. However, there are several advantages to 13C NMR, whether samples can be

Standardization of I-124 by three liquid scintillation-based methods

August 9, 2019
Author(s)
Denis E. Bergeron, Leticia S. Pibida, Brian E. Zimmerman, Jeffrey T. Cessna, Ryan P. Fitzgerald
A solution of 124I was standardized for activity by 4πβ(LS)-γ(NaI) live-timed anticoincidence (LTAC) counting, with confirmatory measurements by triple-to-double coincidence ratio (TDCR) and CIEMAT-NIST efficiency tracing (CNET) liquid scintillation

High-coverage, long-read sequencing of Chinese trio reference samples

June 14, 2019
Author(s)
Justin M. Zook, Nathanael D. Olson, Marc L. Salit, Aaron Wenger, Chunlin Xiao, Robert Sebra
Genome In a Bottle (GIAB) is a consortium hosted by the National Institute of Standards and Technology whose primary objective is the development and characterization of human genomic reference materials. The consortium includes representatives from

An open resource for accurately benchmarking small variant and reference calls

April 1, 2019
Author(s)
Justin M. Zook, Jennifer H. McDaniel, Marc L. Salit, Nathanael D. Olson, Justin M. Wagner
Benchmark small variant calls are required for developing, optimizing and assessing the performance of sequencing and bioinformatics methods. Here, as part of the Genome in a Bottle (GIAB) Consortium, we apply a reproducible, cloud-based pipeline to

Towards quality assurance and quality control in untargeted metabolomics studies

January 3, 2019
Author(s)
Richard Beger, Warwick B. Dunn, Abbas Bandukwala, Bianca Bethan, David Broadhurst, Clary B. Clish, Surendra Dasari, Leslie Derr, Annie Evans, Steve Fischer, Thomas Flynn, Thomas Hartung, David Herrington, Richard Higashi, Ping-Ching Hsu, Christina Jones, Maureen Kachman, Helen Karuso, Gary Kruppa, Katrice Lippa, Padma Maruvada, Jonathan D. Mosley, Ionna Ntai, Clair O?Donovan, Mary Playdon, Daniel Raftery, Daniel Shaughnessy, Amanda Souza, Timothy Spaeder, Barbara Spalholz, Mukesh Verma, Tilman Walk, Ian Wilson, Daniel Bearden, Krista Zanetti
We describe here the agreed upon first development steps and priority objectives of a community engagement effort to address current challenges in quality assurance (QA) and quality control (QC) in untargeted metabolomic studies. This has included (1) a QA

The integration of emerging omics approaches to advance precision medicine: How can regulatory science help?

December 6, 2018
Author(s)
Mark S. Lowenthal, Christina M. Jones, Joan Adamo, Robert Bienvenu, Owen Fields, Soma Ghosh, Michael Liebman, Robert Schuck, Scott Steele
Building on the recent advances in next-generation sequencing, the integration of genomics, proteomics, metabolomics, and other approaches hold tremendous promise for precision medicine. The approval and adoption of these rapidly advancing technologies and

Certification of Standard Reference Material 2365 BK Virus DNA Quantitative Standard

August 29, 2018
Author(s)
Megan H. Cleveland, Natalia Farkas, Kevin M. Kiesler, Blaza Toman, Peter M. Vallone
Standard Reference Material (SRM) 2365 is intended for use in the value assignment of BK virus deoxyribonucleic acid (DNA) to BK DNA controls and standards. A unit of SRM 2365 consists of a well characterized, linearized plasmid, containing BK virus DNA

Conference Report: Representing Ethnic Diversity for Precision Medicine

August 23, 2018
Author(s)
Justin M. Zook, Luke Hickey, Benedict Paten, Robert Sebra, Valerie Schneider
There is a pressing need to better represent ethnic diversity with genomic resources — and to do so in a way that maximizes utility for people working with the Genome Reference Consortium’s human reference genome. That was the theme of a panel discussion

An update on dose calibrator settings for nuclides used in nuclear medicine

June 1, 2018
Author(s)
Denis E. Bergeron, Jeffrey T. Cessna
Most clinical measurements of radioactivity, whether for therapeutic or imaging nuclides, rely on commercial reentrant ionization chambers (“dose calibrators”). The National Institute of Standards and Technology (NIST) maintains a battery of representative

Standardization of 64Cu activity

May 25, 2018
Author(s)
Denis E. Bergeron, Jeffrey T. Cessna, Ryan P. Fitzgerald, Leticia S. Pibida, Brian E. Zimmerman
The complex decay scheme that makes 64Cu promising as both an imaging and therapeutic agent in medicine also makes the absolute measurement of its activity challenging. The National Institute of Standards and Technology (NIST) has completed a primary

Magnetic Resonance Imaging Biomarker Calibration Service: Proton Spin Relaxation Times

May 3, 2018
Author(s)
Michael A. Boss, Andrew M. Dienstfrey, Zydrunas Gimbutas, Kathryn E. Keenan, Jolene D. Splett, Karl F. Stupic, Stephen E. Russek
This document describes the calibration service to measure proton spin relaxation times, T1 and T2, of materials used in phantoms (calibration artifacts) to verify the accuracy of MRI-based quantitative measurements. Proton spin relaxation times are used
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