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Certification of Standard Reference Material 2365 BK Virus DNA Quantitative Standard



Megan H. Cleveland, Natalia Farkas, Kevin M. Kiesler, Blaza Toman, Peter M. Vallone


Standard Reference Material (SRM) 2365 is intended for use in the value assignment of BK virus deoxyribonucleic acid (DNA) to BK DNA controls and standards. A unit of SRM 2365 consists of a well characterized, linearized plasmid, containing BK virus DNA solubilized in 10 mmol/L 2-amino-2-(hydroxymethyl)-1,3 propanediol hydrochloride (Tris HCl) and 1 mmol/L ethylenediaminetetraacetic acid disodium salt (disodium EDTA) pH 8.0 buffer (TE), with 50 ng/μL yeast tRNA added to ensure stability. A unit of the SRM consists of one 0.5 mL tube containing approximately 110 μL of DNA solution. The tube is labeled and is sealed with a screw cap. This publication documents the production, analytical methods, and statistical evaluations involved in realizing this product.
Special Publication (NIST SP) - SP260-191
Report Number


BK virus, DNA, clinical testing


Cleveland, M. , Farkas, N. , Kiesler, K. , Toman, B. and Vallone, P. (2018), Certification of Standard Reference Material 2365 BK Virus DNA Quantitative Standard, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], (Accessed April 16, 2024)
Created August 29, 2018, Updated November 10, 2018