Cleveland, M.
, Farkas, N.
, Kiesler, K.
, Toman, B.
and Vallone, P.
(2018),
Certification of Standard Reference Material 2365 BK Virus DNA Quantitative Standard, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.260-191
(Accessed April 29, 2024)
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Certification of Standard Reference Material 2365 BK Virus DNA Quantitative Standard
Published
Author(s)
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Abstract
Standard Reference Material (SRM) 2365 is intended for use in the value assignment of BK virus deoxyribonucleic acid (DNA) to BK DNA controls and standards. A unit of SRM 2365 consists of a well characterized, linearized plasmid, containing BK virus DNA solubilized in 10 mmol/L 2-amino-2-(hydroxymethyl)-1,3 propanediol hydrochloride (Tris HCl) and 1 mmol/L ethylenediaminetetraacetic acid disodium salt (disodium EDTA) pH 8.0 buffer (TE), with 50 ng/μL yeast tRNA added to ensure stability. A unit of the SRM consists of one 0.5 mL tube containing approximately 110 μL of DNA solution. The tube is labeled and is sealed with a screw cap. This publication documents the production, analytical methods, and statistical evaluations involved in realizing this product.Citation
Special Publication (NIST SP) - SP260-191
Report Number
SP260-191
NIST Pub Series
Pub Type
NIST Pubs
Download Paper
Keywords
BK virus, DNA, clinical testing
Citation
Created August 29, 2018, Updated November 10, 2018