Published: August 29, 2018
Megan H. Cleveland, Natalia Farkas, Kevin M. Kiesler, Blaza Toman, Peter M. Vallone
Standard Reference Material (SRM) 2365 is intended for use in the value assignment of BK virus deoxyribonucleic acid (DNA) to BK DNA controls and standards. A unit of SRM 2365 consists of a well characterized, linearized plasmid, containing BK virus DNA solubilized in 10 mmol/L 2-amino-2-(hydroxymethyl)-1,3 propanediol hydrochloride (Tris HCl) and 1 mmol/L ethylenediaminetetraacetic acid disodium salt (disodium EDTA) pH 8.0 buffer (TE), with 50 ng/μL yeast tRNA added to ensure stability. A unit of the SRM consists of one 0.5 mL tube containing approximately 110 μL of DNA solution. The tube is labeled and is sealed with a screw cap. This publication documents the production, analytical methods, and statistical evaluations involved in realizing this product.
Citation: Special Publication (NIST SP) - SP260-191Report Number:
NIST Pub Series: Special Publication (NIST SP)
Pub Type: NIST Pubs
BK virus, DNA, clinical testing
Created August 29, 2018, Updated November 10, 2018