NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Certification of Standard Reference Material 2365 BK Virus DNA Quantitative Standard

Published

Author(s)

Megan H. Cleveland, Natalia Farkas, Kevin M. Kiesler, Blaza Toman, Peter M. Vallone

Abstract

Standard Reference Material (SRM) 2365 is intended for use in the value assignment of BK virus deoxyribonucleic acid (DNA) to BK DNA controls and standards. A unit of SRM 2365 consists of a well characterized, linearized plasmid, containing BK virus DNA solubilized in 10 mmol/L 2-amino-2-(hydroxymethyl)-1,3 propanediol hydrochloride (Tris HCl) and 1 mmol/L ethylenediaminetetraacetic acid disodium salt (disodium EDTA) pH 8.0 buffer (TE), with 50 ng/μL yeast tRNA added to ensure stability. A unit of the SRM consists of one 0.5 mL tube containing approximately 110 μL of DNA solution. The tube is labeled and is sealed with a screw cap. This publication documents the production, analytical methods, and statistical evaluations involved in realizing this product.
Citation
Special Publication (NIST SP) - SP260-191
Report Number
SP260-191
NIST Pub Series
Special Publication (NIST SP)
Pub Type
NIST Pubs

Download Paper

DOI Link

Keywords

BK virus, DNA, clinical testing
Microbial measurements, Clinical diagnostics, Biopharmaceuticals and Precision medicine

Citation

Cleveland, M. , Farkas, N. , Kiesler, K. , Toman, B. and Vallone, P. (2018), Certification of Standard Reference Material 2365 BK Virus DNA Quantitative Standard, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.260-191 (Accessed October 14, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created August 29, 2018, Updated November 10, 2018
Was this page helpful?