Live quantification of cell viability via neutral red uptake using lens-free imaging
Brian J. Nablo, Jungjoon Ahn, Kiran Bhadriraju, Jong M. Lee, Darwin R. Reyes-Hernandez
We present the quantification of cell viability during neutral red (NR) uptake with a compact lens-free system utilizing two light sources. Conventionally, the NR uptake assay determines cell viability based on the accumulation of NR within lysosomes and is quantified spectrophotometrically after a destructive extraction process. Our NR uptake live imaging system offers in situ monitoring of cell viability while accentuating 3D morphologies, thus diversifying the assays functionality. Combining the low-cost NR uptake assay with the compact architecture of lens-free imaging provides high-throughput, point-of-use, live monitoring of cell viability that eliminates extraction steps and preserves the culture.
Proceedings of Micro Total Analysis Systems 2019
October 27-31, 2019
The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019)