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Bioscience

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Displaying 726 - 750 of 1242

NIST Reference Material Scaffolds Characterized for Cell Response

April 24, 2011
Author(s)
Carl G. Simon Jr., Jeff M. Coles
Reference scaffolds characterized for cell response are being developed for use as a standard for biological characterization of new scaffolds. The need for reference scaffolds to serve as a calibration standard between labs has been identified as critical

Development of Probe Free Mapping of Cell Viability in Hydrogels

April 16, 2011
Author(s)
Joy P. Dunkers, Kaushik Chatterjee, Carl G. Simon Jr.
Diffusion of oxygen in cell/scaffold construct interior is of critical importance to cell viability in constructs of clinically relevant sizes. It has been shown that depletion of oxygen within the scaffold leads to a decrease in cell density and viability

Differences in Polydimethylsiloxane Modification Methods Affect Laminin Deposition and Smooth Muscle Cell Proliferation

April 13, 2011
Author(s)
Joy P. Dunkers, Hae-Jeong Lee, Marvi Matos, Lisa Pakstis, Juan M. Taboas, Steven D. Hudson, Marcus T. Cicerone
When culturing cells on flexible surfaces, it is important to consider extracellular matrix treatments that will remain on the surface under mechanical strain. Here we investigate differences in laminin surfaces that were deposited on oxidized PDMS (plasma

Modulus-Driven Differentiation of Marrow Stromal Cells in 3D Is Independent of Cytoskeletal Integrity

April 11, 2011
Author(s)
Carl G. Simon Jr., Kaushik Chatterjee, Sapun Parekh, Sheng Lin-Gibson, Nicole M. Moore, Marcus T. Cicerone, Marian F. Young
Cell functions such as proliferation, migration, and differentiation are key physiological processes that are influenced by the physiochemical extracellular environment. We report on the effect of three-dimensional (3D) scaffold modulus on human bone

Recent Advances in Electrochemical Glyco-biosensing

April 8, 2011
Author(s)
Rebecca A. Zangmeister, Germarie Sanchez-Pomales
Biosensors based on electrochemical transduction mechanisms have recently made advances into the field of glycan analysis. These glyco-biosensor assays offer simple, rapid, sensitive and economical approaches to the measurement need for rapid glycan

Quantification and Compensation of Unintentional Analyte Aggregation in Electrospray Sampling

March 30, 2011
Author(s)
Mingdong M. Li, Suvajyoti S. Guha, Rebecca A. Zangmeister, Michael J. Tarlov, Michael R. Zachariah
Electrospray (ES) sources are commonly used to introduce non-volatile materials (e.g. nanoparticles, proteins, etc.) in to the gas phase for characterization by mass spectrometry and ion mobility. Recent studies in our group using electrospray ion mobility

Characterization of Hyperspectral Imaging and Analysis via Microarray Printing of Dyes

February 22, 2011
Author(s)
Matthew L. Clarke, Maritoni A. Litorja, David W. Allen, Daniel V. Samarov, Jeeseong Hwang
The application of hyperspectral imaging requires rigorous characterization of the spatial and spectral imaging domains of the system. We present a microarray printing methodology for the testing of absorption or reflectance microscopy measurements. This

NMR Characterization of the Formation Kinetics and Structure of Di-O-Benzylidene Sorbitol Gels Self-Assembled in Organic Solvents

January 19, 2011
Author(s)
David L. VanderHart, Jack F. Douglas, Steven Hudson, Joseph M. Antonucci, Elizabeth A. Wilder
The molecule 1,3:2,4-Di-O-Benzylidene sorbitol (DBS) is a common gelator that forms thermally reversible gels in diverse organic solvents. Solid state 13C and proton NMR techniques, along with electron microscopy, are utilized in an exploratory study of

Detection of Heteroplasmic Mitochondrial DNA in Single Mitochondria

December 17, 2010
Author(s)
Joseph E. Reiner, Rani Kishore, Barbara C. Levin, Thomas Albanetti, Nicholas Boire, Ashley Knipe, Kristian Helmerson, Koren Deckman
Background Mitochondrial DNA (mtDNA) genome mutations can lead to energy and respiratory-related disorders like myoclonic epilepsy with ragged red fiber disease (MERRF), mitochondrial myopathy, encephalopathy, lactic acidosis and stroke (MELAS) syndrome
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