Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Microfluidic Based Contactless DielectrophoreticDevice: Modeling and Analysis

Published

Author(s)

Saugandhika Minnikanti, Darwin Reyes-Hernandez, Ruben C. Aguilar, Joseph Pancrazio, Michael Gaitan, Nathalia Peixoto

Abstract

While there have been many attempts at patterning cells onto substrates, a reliable method for trapping cell clusters and forming cell arrays in a predefined geometry remains to be demonstrated. We intend to develop a multielectrode array platform to initially trap cells via dielectrophoresis (DEP) and to later measure their electrical activity. As a first step toward that objective, here we present an interdigitated microfabricated comb structure. We designed an optimal insulation layer via finite element modeling for maximum dielectrophoretic field strength in solution and minimal cell damage. The microfabricated structure was combined with a microfluidic channel to vertically constrain cell position. With the objective of capturing cells onto the substrate, we here show that there is an optimal thickness of dielectric which limits electrolysis in solution and still allows for sufficient dielectrophoretic force on the cells to pull them onto the surface.

Citation

Minnikanti, S. , Reyes-Hernandez, D. , Aguilar, R. , Pancrazio, J. , Gaitan, M. and Peixoto, N. (2010), Microfluidic Based Contactless DielectrophoreticDevice: Modeling and Analysis, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=907086 (Accessed May 27, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created August 31, 2010, Updated October 12, 2021